STUDIES ON SEED PRIMING AND PRIMED SEED LONGEVITY IN ONION (Allium cepa L.) AND CAPSICUM (Capsicum annuum L.)

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Date
10-09-13
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Seed priming is one of the most simple and economical intervention to enhance the vigour of low quality seeds. Seed ageing affected all vigour related parameters viz; mean seedling length, speed of germination, mean germination time and seedling vigour index. When compared to fresh, ageing reduced the germination to the extent of 26.36 and 22.68% in onion and capsicum, respectively. With respect to effect of seed priming on fresh and aged seeds, results indicated that GA3 followed by KNO3 showed significant effect on all the vigour parameters but more in aged seeds than fresh. In case of onion, there was an increase of germination to the extent of 7, 6, 4% due to GA3, KNO3 and CaCl2 treatments when compared to control. Similar trend was also noticed in capsicum. Since, primed seeds are desiccation sensitive they lose all viable parameters within short span (one month) when stored under ambient condition. However, under MAS, irrespective of the material, temperatures and atmosphere, germination and vigour related parameters were maintained as fresh even after 6 months of storage. Biochemical and molecular studies indicated wide variation in protein/esterase/peroxidase profiles among treatments. However, no change either in DNA quality or quantity and RAPD profiles was noticed. Thus the results clearly revealed that modified atmospheric storage with vacuum and stored at 100C was best for extending the primed seed longevity as evident from both physiological and biochemical parameters. The Protein and enzyme markers can be used as tools to identify loss of vigour during storage.
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animal husbandry, livestock, mastitis, lactation, economic systems, byproducts, yields, biological phenomena, seasons, animal developmental stages
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