FACTORS OF RESISTANCE TO SOYBEAN RUST CAUSED BY Phakopsora pachyrhizi Syd.
Loading...
Date
2007-01-03
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
University of Agricultural Sciences GKVK, Banglore
Abstract
FACTORS OF RESISTANCE TO SOYBEAN RUST CAUSED
BY Phakopsora pachyrhizi Syd.
AMMAJAMMA, R. 2005 P.V. PATIL
MAJOR ADVISOR
ABSTRACT
The present investigation deals with the identification of
biochemical factors of resistance and enzymatic activities as influenced
by rust caused by Phakopsora pachyrhizi Syd. in two resistant (EC
241778 and EC 241780) and two susceptible genotypes (JS 335 and
DSb 1) of soybean at 45 and 75 DAS. Also the characterization of these
genotypes through DNA fingerprinting by RAPD technique was carried
out.
The study indicated that at both the intervals, the resistant
genotypes had more of total phenols, sugars (reducing, non-reducing
and total sugars) and chlorophyll content (Chl-a, Chl-b and total
chlorophyll) than susceptible genotypes. But due to infection the
per cent increase in total phenol was maximum in susceptible
genotypes. However the quantity of sugars and chlorophyll content were
decreased in diseased leaf than healthy ones.
Analysis on isozyme pattern revealed that, only at 75 DAS both
peroxidase and polyphenol oxidase shown polymorphic bands, which
indicated the need based synthesis of isozymes.
The activities of phenylalanine ammonia lyase and tyrosine
ammonia lyase were found similar. However from initial to final stage the
activity was found increasing in resistant genotypes where as decreasing
in susceptible genotypes.
RAPD profiles for four genotypes were generated with 60 random
primers out of these OPB-8 and OPB-17 shown polymorphic bands in
both resistant genotypes but absent in susceptible genotypes. Similarly
other six primers shown polymorphism in both the susceptible
genotypes but not in resistant genotypes. Therefore these primers can be
tested with large number of genotypes to identify the consistent cosegregation
between polymorphic band and disease resistance /
susceptibility to utilize them in marker assisted selection programme.
Description
Keywords
null