CHARACTERIZATION OF CULTURABLE BACTERIAL ENDOPHYTES OF CHRYSANTHEMUM (Dendranthema grandiflora Tzvelev) AND THEIR EFFICACY FOR PLANT GROWTH PROMOTION

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dc.contributor.advisorCHAUHAN, ANJALI
dc.contributor.authorSHARMA, SHILPA
dc.date.accessioned2020-01-03T08:54:53Z
dc.date.available2020-01-03T08:54:53Z
dc.date.issued2019-11
dc.description.abstractABSTRACT Chrysanthemum (Dendranthema grandiflora Tzvelev) belongs to family Asteraceae and is a popular flower crop suitable for both pot culture and bedding purposes. The quality of flowers is greatly influenced by the quantity as well as sources of nutrients. Presently, these nutrients are supplied through chemical fertilizers. The escalating prices of chemical fertilizers and their indiscriminate use has not only adversely affects the soil health and environment but also reduces the productivity of crops. The situation emphasized the need for developing alternate production system that is eco-friendly and is more judicious in maintaining soil health. So, the present investigations were carried out to characterize and evaluate the effects of PGPB isolated from chrysanthemum plant (roots, stem and leaf) samples grown under organic and commercial cultivation. A total of 143 purified isolates were selected on the basis of PGP traits and morphological characterization. From the total, 44 (16 organic and 28 inorganic) isolates were selected on the basis of their efficacy to have maximum plant growth promoting traits like P-solubilization, growth on nitrogen free medium, siderophore, auxin, HCN production and antagonism against Pythium ultimum, Rhizoctonia solani and Fusarium oxysporumunderlaboratoryconditions.The genetic diversity based on 16S r DNA sequence analysis and BLASTn search revealed the identity of 44 strains belonging to diverse genera viz.,Bacillus, Pseudomonas, Stenotrophomonas, Lysinibacillus, Micrococcus, Streptomyces, Pantoea, Klebsiella, Phyllobacterium, Serratia, Microbacterium, Cellulosimicrobium, Arthrobacter and Staphylococcus. From selected 44 isolates, seven isolates viz. Stenotrophomonas pavanii strain SRO8, Bacillus subtilis strain RDO10, Serratia marcescens strain N2S14, Bacillus subtilis strain N1S25, Serratia nematodiphila strain N1S23, Pseudomonas aeruginosa strain N2S6 and Bacillus subtilis strain N2S18 along with consortium of reference strains (Bacillus licheniformis strain KS_5+Bacillus subtilis strain KS_6) were selected for pot trial to study their efficacy for growth and development of chrysanthemum. In the pot trial experiment two best isolates were selected with different levels of NP fertilizers along with two efficient isolates (KS5 and KS6) and used for field trial using two varieties of chrysanthemum viz ‘Ajay’ and ‘Purnima’. The application of 70% RD NP + Bacillus subtilis strain RDO10 and 70% RD NP + Pseudomonas aeruginosa strain N2S6 registered maximum increase in all plant parameters i.e. plant height (cm), number of side shoots per plant, days taken to flowering, number of flowers per plant, flower size (cm), duration of flowering (days) and vase life (days). The inoculation of PGPB has also registered an increase in NPK uptake and available NPK. Thus, the selected bacterial endophytes has good prospects to be used as biofertilizer, biostimulant and bioprotectant for chrysanthemum.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810139277
dc.keywordsChrysanthemum, bedding, chemical fertilizers,nutrientsen_US
dc.language.isoenen_US
dc.pages156+XVen_US
dc.publisherUHF,NAUNIen_US
dc.subBasic Sciencesen_US
dc.subjectnullen_US
dc.themeChrysanthemum, bedding, chemical fertilizers,nutrientsen_US
dc.these.typePh.Den_US
dc.titleCHARACTERIZATION OF CULTURABLE BACTERIAL ENDOPHYTES OF CHRYSANTHEMUM (Dendranthema grandiflora Tzvelev) AND THEIR EFFICACY FOR PLANT GROWTH PROMOTIONen_US
dc.typeThesisen_US
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