STUDIES ON PREVALENCE, HAEMATO – BIOCHEMICAL AND HISTOPATHOLOGICAL ASPECTS OF FASCIOLOSIS IN SLAUGHTERED BUFFALOES
| dc.contributor.advisor | Dr. J. J. Hasnani | |
| dc.contributor.author | Suchit S. Pandya | |
| dc.date.accessioned | 2018-01-04T09:20:39Z | |
| dc.date.available | 2018-01-04T09:20:39Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | A total of 760 faecal and 701 liver samples were collected at Anand district out of which 151 faecal and 164 liver samples were found positive with the seasonal prevalence of 12.83% (summer), 21.54% (monsoon) and 23.95% (winter) for the faecal samples and 15.34% (summer), 24.34% (monsoon) and 28.62% (winter) for the liver samples. A total of 792 faecal and 743 liver samples were collected at Ahmedabad district out of which 163 faecal and 157 liver samples were found positive with the seasonal prevalence of 16.93% (summer), 21.83% (monsoon) and 22.61% (winter) for the faecal samples (Table 6, Figure 7) and 17.60% (summer), 22.08% (monsoon) and 23.37% (winter) for the liver samples. The overall prevalence rate was found to be 19.87% (151) and 23.39% (164) for the faecal and liver samples at Anand district and 20.58% (163) and 21.13% (157) for faecal and liver samples at Ahmedabad district. Fasciolosis affects the haematological values of the host. The haematological parameters viz. haemoglobin (Hb), packed cell volume (PCV), total erythrocyte count (TEC), total leukocytes count (TLC), different leukocyte count (DLC) were studied from Fasciola infected and non-infected buffaloes. A total number of 50 Fasciola infected and 50 non-infected blood samples were collected and studied for haematological parameters. Following results were found in the study. The infected buffaloes showed a significant reduction in the mean Hb. (7.29 ± 0.21 g/dl), TEC (5.15 ± 0.19 106/μl), PCV (24.82 ± 0.88 percent), lymphocyte (47.59 ± 0.75 percent) and increased TLC (12.78 ± 0.37 103/ μl), neutrophil (42.43 ± 0.84 percent) and eosinophil (8.29 ± 0.26 percent). The monocytes level decreased and basophils levels increased non-significantly in infected buffaloes and recorded as, 2.78 ± 0.16 and 0.38 ± 0.05 percent, respectively. A total number of 50 infected and 50 non-infected serum samples were collected and studied. The biochemical profile included Total protein, Albumin, Globulin, A:G ratio, Alkaline Phosphatase (AKP), Acid Phosphatase (ACP), Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) and Total Bilirubin levels of infected and non-infected buffaloes and the results are presented as follows. Significantly reduction in the values of total serum protein (7.37 ± 0.09 g/dl), total albumin (1.92 ± 0.06 g/dl) and A:G ratio (0.36 ± 0.02) and increased in AST/ SGOT (305.82 ± 13.40 IU/L), total globulin (5.44 ± 0.09 g/dl), ALT/ SGPT (79.25 ± 2.24 IU/L), ACP (4.30 ± 0.20 IU/L), alkaline phosphatase (189.50 ± 6.31 IU/L) and total bilirubin (1.93 ± 0.13 IU/L) level reported. The histopathological changes in Fasciolosis in buffaloes were observed during study period. A total of 30 positive fasciola infected liver was collected from slaughter houses of Anand and Ahmedabad districts. The gross lesions in Fasciolosis were, the body cavities of slaughtered buffaloes were found to contain large amount of straw colored fluid with fibrin flakes. There was oedema in brisket and dewlap region and petechial haemorrhage in muscles in some of the buffaloes. Liver was found severely affected, enlarged, covered with rust colored patches, showing haemorrhagic tracts, perforation and presence of large number of immature flukes in the parenchyma and at the opening of the bile duct. At places blood filled cavities were also seen in the parenchyma. Hundreds of immature and mature flukes were recovered after squeezing and tearing of liver. Gross lesions were confined to the liver which was enlarged, haemorrhagic, highly congested and had wide-spread greyish creamy deposits on its surface. Migrating flukes caused extensive destruction of liver parenchyma marked with haemorrhages. The microscopic lesions in Fasciola infected liver and bile duct were, The sections of liver showed haemorrhagic tracts, wide-spread area of necrosis and haemorrhages. It also revealed focal necrosis with heavy infiltration of inflammatory cells. Portal triad area revealed proliferation of fibrous tissue. Hepatic cells showed degenerative changes and mild fatty changes. There were large numbers of multiple haemorrhagic tracts made up of erythrocytes and degenerating hepatic cells with polymorphs, eosinophils and mononuclear cells. Bile ducts showed hyperplasia, desquamation and degeneration of epithelium. The epithelium of bile duct both close to and distal to the sites of fluke penetration was highly hyperplastic and thickened with numerous eosinophils and mononuclear cells infiltration into the lamina propria. F. gigantica had a less prominent shoulder and having 25-35 mm body length. The ventral sucker is situated at the level of the shoulder and tegument is armed with sharp spines. Pharynx is situated just after the oral sucker. The testes are much branched and filling the median plane of the fluke. Uterus is situated posterior to the ventral sucker and anterior to the testes and ovary is situated right of the middle of the fluke. The digestive tract of the fluke covered with two type of epithelium viz; tegumental and caecal type of epithelium. The major portion of the digestive tract is lined by caecal type of the epithelium. Mehlis gland was close to ovary. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810038770 | |
| dc.keywords | PREVALENCE, HAEMATO – BIOCHEMICAL, HISTOPATHOLOGICAL, FASCIOLOSIS, SLAUGHTERED BUFFALOES | en_US |
| dc.language.iso | en | en_US |
| dc.publisher | AAU, Anand | en_US |
| dc.research.problem | STUDIES ON PREVALENCE, HAEMATO – BIOCHEMICAL AND HISTOPATHOLOGICAL ASPECTS OF FASCIOLOSIS IN SLAUGHTERED BUFFALOES | en_US |
| dc.sub | Veterinary Parasitology | en_US |
| dc.subject | veterinary science, parasitology | en_US |
| dc.subject | study | en_US |
| dc.theme | PREVALENCE, HAEMATO – BIOCHEMICAL AND HISTOPATHOLOGICAL ASPECTS OF FASCIOLOSIS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | STUDIES ON PREVALENCE, HAEMATO – BIOCHEMICAL AND HISTOPATHOLOGICAL ASPECTS OF FASCIOLOSIS IN SLAUGHTERED BUFFALOES | en_US |
| dc.type | Thesis | en_US |