ISOLATION CHARACTERIZATION AND PATHOGENICITY OF Escherichia coli SEROTYPES OF COLIBACILLOSIS OF CHICKEN
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Date
2008-10
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Sri Venkateswara Veterinary University, TIRUPATI – 517 502,A.P
Abstract
ABSTRACT :
Colibacillosis is one of the principal causes morbidity and mortality in chickens. Among the various diseases which cause impediment to the development of poultry scenario, colibacillosis is responsible for major economic loss.
In order to understand the serotypes available locally in the region, to have an idea about their pathogenicity, band pattern of fimbrial protein and antibiogram patterns, the present study was taken up.
In the present study a total of 50 samples were screened and 25 (50%) isolates of E. coli identified from in and around Tirupati poultry farms. Confirmation of etiological agent was carried out by standard morphology, biochemical, and sugar fermentation reactions. The isolates were typed at CRI, NSEC, Kasauli and serotypes identified were the O138(3) and O2(2) individual serotypes were O5, O28, O60, O69, O80 and O123. The Hemagglutination study was performed on different species of erythrocytes viz., guinea pig, mice, rabbit, cattle, goat, sheep and chicken and different E. coli serotypes revealed different hemagglutination patterns. Mannose Sensitive Hemagglutination also performed to identify the type I fimbriae which were responsible for attachment to the upper respiratory and gastrointestinal epithelial cells and establish the infections.
Fimbrial protein was extracted from E. coli serotypes and subjected to SDS-PAGE that revealed a protein band of 18 kDa.
Pathogenicity studies on chicks revealed that, six serotypes were able to exhibits typical lesions like airsacculitis, pericarditis, perihepatitis and peritonitis.
To differentiate the invasive type of E. coli from non- invasive type, Congo red binding was employed which revealed rough red color colonies and white colorless colonies that indicated invasive type and non invasive type of serotypes respectively.
Antibiogram pattern studies revealed that the drug chloremphenicol was most suitable for colibacillosis, followed by streptomycin, ciprofloxacin and gentamicin.
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Keywords
Escherichia coli; E. Coli; Colibacillosis; CHICKEN