Phylogenetic Analysis of 4b Core Protein Gene of Fowlpox Virus Field Isolates and Commercial Vaccines of Indian Origin
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Date
2019-03
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Abstract
The highly conserved P4b gene Was ampli-
fied from two field isolates (FWPV-W1 and
FWPV-W2) and two commercial vaccines
(FWPV-G and FWPV-V) of fowlpox virus and
was subjected nucleotide sequencing. The nucle-
otide sequence (512 nucleotides) showed 100%
sequence similarity in BLAST analysis with
other FWPV sequences available in GenBank.
Phylogenetic analysis of partial P4b gene (495
nucleotides) sequences of both the field isolates
and both the commercial vaccines was done
using Neighbour-joining method with 1000
bootstrap replications. In the P410 partial gene
based phylogenetic tree, both the field isolates
and both the commercial vaccines were grouped
under sub-clade A1 along with other fowlpox
viruses.
Description
TNV_IVJ_2019_96(3)34-36
Keywords
Veterinary Science