STANDARDIZATION OF TRANSFORMATION PROTOCOL AND EXPRESSION OF HEPATITIS B SURFACE ANTIGEN (HBsAg ) IN Coleus forskholii
| dc.contributor.advisor | RAMANJINI GOWDA, P H | |
| dc.contributor.author | DESHMUKH SNEHAL, SANJAYRAO | |
| dc.date.accessioned | 2016-11-14T08:35:23Z | |
| dc.date.available | 2016-11-14T08:35:23Z | |
| dc.date.issued | 2013-08-27 | |
| dc.description.abstract | Hepatitis B Virus (HBV) infection remains a major worldwide infectious disease with serious long-term morbidity and mortality. It is estimated that two billion people have been infected with the virus, and more than 360 million have chronic HBV infections. The effective way to control the virus is to take HBV vaccine. Hepatitis B surface antigen (HBsAg) is an effective protective antigen suitable for vaccine development for prevention of this disease. Hence, plant-based production of vaccine for hepatitis B may be an economically feasible alternative. The present study lays emphasis on production of a recombinant protein/vaccine (HBsAg, Hepatitis B surface antigen) in Coleus forskohlii against hepatitis-B disease through Agrobacterium mediated gene transfer. MS basal media with 6mg/L BAP and 0.4mg/L IAA was effective to induce a good callus as well as multiple shoot production from leaf explant. The shoots were also rooted on same MS basal media. The control of Agrobacterium overgrowth was effective in OD dilution of 1:40 and 1:50with OD value of 0.6, immersion time of 2 minutes and co-cultivation period of one day. The transformed plants were confirmed by Polymerase Chain Reaction (PCR) analysis which has shown a band size of 681bp. Expression of HBsAg was confirmed by Sodium Dodecyl Sulphate (SDS) and DOT-BLOT. The expected protein band size of 24 KDa on gel confirmed expression of HBsAg S-protein in transgenic plants. Since Coleus forskohlii is a medicinal plant and nature of propagation is vegetative, it is advantageous to produce recombinant HBsAg S-protein in this plant. | en_US |
| dc.identifier.other | TH-10522 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/85299 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Bangalore | en_US |
| dc.sub | Biotechnology | en_US |
| dc.subject | planting, proteins, biological phenomena, genes, genetic processes, dna, diseases, transgenics, concentrates, bacteria | en_US |
| dc.title | STANDARDIZATION OF TRANSFORMATION PROTOCOL AND EXPRESSION OF HEPATITIS B SURFACE ANTIGEN (HBsAg ) IN Coleus forskholii | en_US |
| dc.type | Thesis | en_US |