Studies on validation of molecular markers linked to wilt resistance, polymorphic information content (PIC) and transferability of functional markers in chickpea (Cicer arietinum L.)
| dc.contributor.advisor | RAVIKUMAR, R L | |
| dc.contributor.author | JAYALE ASHWIN, SANJAYRAO | |
| dc.date.accessioned | 2016-11-14T12:59:45Z | |
| dc.date.available | 2016-11-14T12:59:45Z | |
| dc.date.issued | 2013-07-27 | |
| dc.description.abstract | Chickpea (Cicer arietinum L.) is one of the most important pulse crops in the Indian subcontinent and the Mediterranean regions. Chickpea wilt, caused by Fusarium oxysporum f. sp. Ciceris, is the most important biotic factor reducing productivity in India. Allele specific associated primers (ASAP) i.e.-27700 linked H1 locus and SSR markers TA- 72, TR-20, NCPGR-74, and H4G11 linked to H2 locus were reported by earlier workers. The validation of these markers linked with wilt resistant loci was carried out by studying 11 diverse chickpea lines for wilt reaction and markers. The marker CS-27700 was found to be consistent with the phenotypic reaction and function across genotypes but SSR markers were not consistent. The presence of this dominant marker indicates susceptibility to wilt. In addition, 60 newly developed transcriptome based SSR markers were studied for their polymorphic information content (PIC) and cross-species transferability across different pulses like horse gram, cowpea, pigeon pea, black gram and soybean. All the 60 primers studied gave amplification in all the 11 chickpea genotypes. Among which, 50 were monomorphic and 10 were polymorphic. The PIC value ranged from 0.151 to 0.372. Among other pulses, the highest amplification rate of these SSRs were found in horse gram (30.0%) followed by cowpea (20.0%), pigeon pea (11.6%), soybean (10.0%), black gram (10.0%). The results indicate that genic SSR markers are less polymorphic but highly transferable among other legume species. Chickpea SSR markers are useful to develop genomic resources in orphan crops like horse gram, black gram and cowpea. | en_US |
| dc.identifier.other | TH-10539 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/85390 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Bangalore | en_US |
| dc.sub | Plant Biotechnology | en_US |
| dc.subject | diseases, fungi, chickpeas, biological development, planting, biological phenomena, genotypes, extraction, irrigation, pathogens | en_US |
| dc.these.type | Ph.D | |
| dc.title | Studies on validation of molecular markers linked to wilt resistance, polymorphic information content (PIC) and transferability of functional markers in chickpea (Cicer arietinum L.) | en_US |
| dc.type | Thesis | en_US |