Studies on validation of molecular markers linked to wilt resistance, polymorphic information content (PIC) and transferability of functional markers in chickpea (Cicer arietinum L.)
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Date
2013-07-27
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Chickpea (Cicer arietinum L.) is one of the most important pulse
crops in the Indian subcontinent and the Mediterranean regions.
Chickpea wilt, caused by Fusarium oxysporum f. sp. Ciceris, is the most
important biotic factor reducing productivity in India. Allele specific
associated primers (ASAP) i.e.-27700 linked H1 locus and SSR markers
TA- 72, TR-20, NCPGR-74, and H4G11 linked to H2 locus were reported by
earlier workers. The validation of these markers linked with wilt resistant
loci was carried out by studying 11 diverse chickpea lines for wilt
reaction and markers. The marker CS-27700 was found to be consistent
with the phenotypic reaction and function across genotypes but SSR
markers were not consistent. The presence of this dominant marker
indicates susceptibility to wilt. In addition, 60 newly developed
transcriptome based SSR markers were studied for their polymorphic
information content (PIC) and cross-species transferability across
different pulses like horse gram, cowpea, pigeon pea, black gram and
soybean. All the 60 primers studied gave amplification in all the 11
chickpea genotypes. Among which, 50 were monomorphic and 10 were
polymorphic. The PIC value ranged from 0.151 to 0.372. Among other
pulses, the highest amplification rate of these SSRs were found in horse
gram (30.0%) followed by cowpea (20.0%), pigeon pea (11.6%), soybean
(10.0%), black gram (10.0%). The results indicate that genic SSR
markers are less polymorphic but highly transferable among other
legume species. Chickpea SSR markers are useful to develop genomic
resources in orphan crops like horse gram, black gram and cowpea.
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Keywords
diseases, fungi, chickpeas, biological development, planting, biological phenomena, genotypes, extraction, irrigation, pathogens