STUDIES ON NATIVE MICROBIAL BIOAGENTS FORMULATIONS IN BELL PEPPER DISEASE MANAGEMENT
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Date
2023-12-11
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UHF,NAUNI
Abstract
In present investigations, the effect of incubation period, incubation temperatures, dilution factor and their interactions on colony forming units were studied and it was noticed that if Trichoderma atroviride is incubated for four days at 29±1°C then the desired number of cfu count can easily be obtained and quantified at dilution factor 1012. Similarly, when Bacillus subtilis is incubated for three at 31±1°C, then the desired number of cfu count can easily be obtained and quantified at 1014 dilution factor for the production of formulations. The shelf-life trends obtained during this study up to 90 days were indicative that at 4°C, the formulations retained comparatively higher number of viable propagules as compared to room temperature. It was evident from the research that the storability and retention of cfu viability is directly proportional to the CMC concentration in talc-based formulations, and inversely proportional to the talc concentration. It has also been documented that using glycerol as a stabilizer increased the shelf life of Trichoderma atroviride and Bacillus subtilis liquid formulation. Hence it was investigated that Trichoderma atroviride talc-based formulation with 1:2 talc and 0.75 per cent CMC was evaluated as the best formulation as it retained 20.8×107 cfu/g after 90 days of storage, whereas, the best talc-based formulation of Bacillus subtilis was having 1:25 talc ratio and 0.75 percent CMC as it retained cfu count of 34.7×109 cfu/g after 90 days of storage. The best formulated product of Trichoderma atroviride and Bacillus subtilis had 9 per cent and 7.5 per cent glycerol concentration, respectively, as it retained more cfu count than the desired cfu count of 2×106 and 2×108 cfu/ml even after 90 days of storage. It was also concluded that the native microbial bioagent formulations not only helped in mitigating the losses due to soil-borne diseases but also helped in improving plant growth because the microbial consortium of liquid formulation of (Trichoderma atroviride + Bacillus subtilis) showed minimum disease incidence (44.44%).