MORPHO-PHYSIO-BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF SELECTED POTATO (Solanum tuberosum L.) VARIETIES UNDER DROUGHT STRESS
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Date
2020-12
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SARDAR VALLABHBHAI PATEL UNIVERSITY OF AGRICULTURE AND TECHNOLOGY, MEERUT- 250110 (U.P.),
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ABSTRACT
Name: Vyankatesh Dhanraj Bagul Id. No.: PG/4357/18-19
Degree: M.Sc. (Ag.) Major: Agriculture Biotechnology
College: College of Agriculture Minor: Genetics and Plant Breeding
Advisor: Dr. R. S. Sengar
Thesis title: “Morpho-physio-biochemical and molecular characterization of selected
potato (Solanum tuberosum L.) varieties under drought stress”.
Potato (Solanum tuberosum L.) is considered as an important cash crop of the world
and also known as apple of soil. It provides food for millions of people across the globe with
being the 4th largest produced crop. Drought is one of the major stress factors affecting the
growth and development of potato plants. In this study, we have selected 5 Indian potato
varieties and supplemented them with 2 different drought treatments viz., allowed them to
undergo heavy drought stress and application of aquasorb at the time of sowing at the rate
of 30Kg/ha. Both the treatments were subjected for 15 days of drought stress by water
withholding at tuber initiation stage which is known to be the most vulnerable stage due to
drought stress. The experiment was conducted in CRD pattern using experimental pots at
the rain out shelter of Department of Agriculture Biotechnology. Various morpho-physiobiochemical
parameters regarding drought stress responses of plant were selected, measured
and compared within the varieties after the drought stress period was completed. Also, seven
drought related gene specific marker were synthesized using bioinformatics tools and were
used for molecular characterization of selected potato varieties.
The study suggests that, drought has negatively impacted on all the varieties used in
the experiments regardless of their stress response nature. The impact of drought stress
varied among the plant varieties, where K. Thar-1, a drought tolerant variety under drought
stress was found significantly superior over K. Pukhraj, K. Bahar, K. Chipsona-1 and K.
Chipsona-1 in the morpho-physio-biochemical parameters namely no. of compound leaves
per plant (10.3), no. of tubers per plant (18.67), weight of tubers per plant (238.89gm), top
biomass weight per plant (336.83gm), root biomass weight (20.7gm), average stolon length
(10.8cm), chlorophyll content(31.80), leaf area index (1.52), relative water content
(56.45%), sucrose content (381.49mg), reducing sugar content (73.87mg), proline content
(28.83μmols), catalase activity (24.57U), peroxidase activity (61.45ΔA/min) and abscisic
acid content (256.49pmol). In aquasorb treated drought stressed conditions, all the varieties
shown great resilience and were found to be significantly superior over drought stressed
conditions. This experiment suggest that Aquasorb can help to minimize the losses occurred
in crop production. Molecular characterization of these selected potato varieties gave three
polymorphic bands and seven monomorphic bands at an average pf 1.43 bands per primer.
The dendrogram using bioinformatics tools explains inter-varietal hierarchical relationship.
(R. S. Sengar) (Vyankatesh Bagul)
Advisor Author