Physiological and anatomical reaction of grape (Vitis vinifera L.) genotypes to anthracnose

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dc.contributor.advisorNirmaljit Kaur
dc.contributor.authorMurria, Sarah
dc.date.accessioned2018-01-29T11:09:30Z
dc.date.available2018-01-29T11:09:30Z
dc.date.issued2017
dc.description.abstractThe present investigation was undertaken with the objective to screen five grapevine varieties against anthracnose caused by Elsinoe ampelina (de Bary) Shear under field conditions; and to evaluate the anatomical, physiological and biochemical characteristics in relation to reaction of the grapevine varieties to the disease. On the basis of Percent disease incidence (PDI) worked out in the leaves, the variety Punjab MACS Purple was characterized as resistant, Pusa Navrang and Flame Seedless as moderately resistant and Beauty Seedless and Perlette as susceptible varieties. The stomatal characteristics viz., stomatal size and stomatal index were significantly high in most susceptible variety Perlette. The variety Punjab MACS Purple which is resistant to the pathogen had compact spongy mesophyll and visibly high trichome density. Significant Pearson correlation coefficient has been found between PDI and stomatal size and stomatal index. The physiological and biochemical constituents viz., chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, total soluble sugars, total soluble proteins, free amino acids, total phenols, ascorbic acid, proline, α-tocopherol and MDA content was quantified from the healthy and infected leaves of the five grapevines varieties. The chlorophyll a, chlorophyll b and total chlorophyll were higher in the healthy leaves as compared to the infected ones. These pigments were high in the resistant variety and low in the susceptible variety. The carotenoids were higher in the healthy leaves as compared to the infected ones. This was high in the susceptible variety and low in the resistant variety. The total soluble sugars, total soluble proteins, free amino acids, total phenols, ascorbic acid, proline and α-tocopherol were significantly higher in the resistant variety Punjab MACS Purple and minimum in the susceptible variety Perlette whereas the MDA content was low in the Punjab MACS Purple and maximum in the Perlette. The activity of the peroxidase and polyphenol oxidase was significantly higher in the infected leaves as compared to their healthy counterparts in all the grape varieties. Among different varieties, peroxidase and polyphenol oxidase was maximum in Punjab MACS Purple and minimum in Perlette. Highest Pearson correlation coefficient of PDI has been observed and chlorophyll b followed by total phenols. The characteristics viz., stomatal size, stomatal index, pigments and total phenols can therefore be used as markers for evaluating grapevine genotypes against Elsinoe ampelina.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810040010
dc.keywordsAnthracnose, Elsinoe ampelina, enzymes, phenols, pigments, stomatal characteristicsen_US
dc.language.isoenen_US
dc.pages61en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemPhysiological and anatomical reaction of grape (Vitis vinifera L.) genotypes to anthracnoseen_US
dc.subBotanyen_US
dc.subjectnullen_US
dc.themePhysiological and anatomical reaction of grape (Vitis vinifera L.) genotypes to anthracnoseen_US
dc.these.typeM.Scen_US
dc.titlePhysiological and anatomical reaction of grape (Vitis vinifera L.) genotypes to anthracnoseen_US
dc.typeThesisen_US
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