Reproductive biology, molecular profiling and biochemical analysis of Terminalia chebula (Retz.)
| dc.contributor.advisor | N. B. Singh | |
| dc.contributor.author | Sankanur, Mahantappa | |
| dc.date.accessioned | 2017-06-03T11:18:29Z | |
| dc.date.available | 2017-06-03T11:18:29Z | |
| dc.date.issued | 2013 | |
| dc.description.abstract | The present investigation entitled “Reproductive biology, molecular profiling and biochemical analysis of Terminalia chebula (Retz.)” was carried out at Regional Horticultural Research Station, Jachh, Kangra and in the Department of Tree Improvement and Genetic Resources, Dr. Y. S. Parmar University of Horticulture & Forestry Nauni, Solan (HP) during 2010-2013. Reproductive biology of six T. chebulagenotypes revealed that Kothi (G 1), Paragpur 2 (G 3 ), Kallar (G4), Bhella (G 5) and Tamber (G 6 ) initiated most of their phenological events earlierthan Paragpur 1 (G 2 ) genotype. The flower buds busted after the leaves started to emerge for all the genotypes. Among genotypes each spike is on an average 6.79 ± 0.45 cm and produces 40.67 ± 3.36 flowers acropetally over a period of 6–7 days. On thebasis of sizes and development stages, the flower buds were assorted into seven different stages. The stigma protrudes out of the calyx during the mature bud stage. It is receptive since then and continues until the evening of the 3 rd day. The pollen–ovule ratio is 10,890:1. Anin-vitropollen germination percentage of freshly collectedpollen was higher in 30% sucrose with Brewbaker and Kwack’s medium (BKM). Fresh pollen viability percentages were generally high (above 70 per cent) for most of the genotypes. -20 0 C temperature with controlled humidity was found effective in long term storage of pollens for breeding programmes. The manual pollinations performed for autogamy, geitonogamy and parthenocarpy did not set fruit. Whereas, those performed for the xenogamous mode set fruit (65.76 ± 10.4%) and in open-pollinations the fruit set was 7.83 ± 0.78%. The flowers were foraged during daytime by 42 species of insects representing bees, wasps, bugs, flies, butterflies and beetles. Crossability pattern studies revealed thatgenotypes under study were cross compatible shouldbe involved in intraspecific breeding programme. In all the fourteen successful crosses between different genotypes were obtained. Per cent successful cross was highest in cross Bhella (G5) × Paragpur 1 (G 2) that was 2.14 per cent. The data for various nursery traits viz., collar diameter, plant height and number of leaves for various crosses was recorded when seedlings were 8 months old. Collar diameter for various crosses ranged between 1.36 mm to 3.38 mm. maximum value for collar diameter was recorded for cross Bhella (G5) × Kallar (G 4 ) i.e.3.38 mm. The maximum plant height was 20.80 cm which was recorded for cross Paragpur 2 (G 3) × Paragpur 1 (G2 ). The maximum number of leaves was found in the cross Paragpur 2 (G 3 ) × Paragpur 1 (G 2 ). Among the six genotypes various qualitative morphological descriptors studied viz.,crown of tree, branching type, bark colour, leaf colour, arrangement, shape, tip shape, base, margin, flower type, flower colour, fruit base and tip and seed colour did not show any variation at all except forthat fruit shape. Fruit samples collected from sixdifferent genotypes showed variation in their shapes, bases, colours, physical dimensions and chemical characteristics. Fruit shapes were of obovoid to ellipsoidal obovoid. While, the fruit base were, varied from round - broad. Fruit colour were Yellow green group – 144 A and 144 B. Fruits from Paragpur 1 (G 2 ) showed maximum values of fresh fruit weight (44.41 g), fresh pulp weight/fruit (42.08 g), fresh fruit length (6.46 cm), fresh pulp/kernel ratio (18.06) and dry pulp/kernel ratio (5.39). The present study confirms the need for domestication of T. chebulato be based on two ideotypes, one for fruit flesh [Paragpur 1 (G 2 )] and the other for seed trait [Paragpur 2 (G 3)]. Twenty five RAPD and twelve ISSR primers were effective in revealing polymorphisms among different genotypes of T. chebula. RAPD exhibited 96.76 per cent polymorphism among six genotypes, out of the total, 124 scorable bands, 120 showed polymorphism and 4 bands exhibited monomorphism. Total numbers of amplified and polymorphic fragments generated per ISSR primer revealed 97.92 per cent polymorphism among genotypes. Genotype Paragpur 2 (G 3) came as outliner as revealed by both ISSR study and combined data (RAPD and ISSR). Crossability pattern studies revealed that genotypes under study were cross compatible should be involved in intraspecific breeding programme. The overall profile of various macro and micro minerals as well as other chemical constituents shows T. chebula fruits as highly nutritious. T. chebula fruits should be regularly, used either in the raw form or in the form of 'Jams' and 'Murebba’ so that these fruits become an important part of our diet to supplement human dietary requirements. Signature of Major | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810017179 | |
| dc.language.iso | en | en_US |
| dc.publisher | DYSPU | en_US |
| dc.sub | Forestry | |
| dc.subject | Terminalia chebula, Reproductive biology, Molecular profiling | en_US |
| dc.these.type | Ph.D | |
| dc.title | Reproductive biology, molecular profiling and biochemical analysis of Terminalia chebula (Retz.) | en_US |
| dc.type | Thesis | en_US |
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