PHENOTYPIC AND MOLECULAR ANALYSIS OF Helicoverpa armigera (Hubner) ON SELECTED HOSTS
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Date
2006-12-30
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University of Agricultural Sciences GKVK, Banglore
Abstract
Investigations on the phenotypic and molecular analysis of Helicoverpa armigera (Hubner)
on selected host plants were undertaken at the Institute of Agricultural Biotechnology and in
Department of Agricultural Entomology, Dharwad during 2004-2005 revealed significant variation
between the populations from ten different host plants. Totally, ten different body colour morphs
were encountered across different host plants, with an average numbers of 6.1. Greenish (24.8%)
and dark green (16.5%) colour morphs were found to be dominant over other colour morphs.
Significantly maximum individuals with no pigmentation (54.40%) on the lateral bands followed by
continuous (27.90%) and discontinuous bands (17.70%) were noticed. Mean frequency of
pigmentation on thorax and abdomen indicated an average of 6.4, 26.6 respectively across the
populations. Mean intensity of pigmentation on thorax was 1.18 and on abdomen it was 1.67. The
populations from chickpea, pigeonpea and cotton were found to possess significantly higher
phenotypic attributes. WhUe, population from niger and sorghum recorded least morphometric
traits. Based on simple matching coefficients, a genetic similarity matrix was constructed to assess
the genetic relatedness. Similarity coefficients ranged from 0.25 to 0.82 among populations. The
minimum genetic similarity was 0.19 between groundnut and sunflower populations. The highest
genetic similarity of 0.72 was evident between the populations of safQower. Overall, it was evident
that majority of the H. armigera from crops viz., niger, safflower, greengram, abutilon and lagasca
clustered together. Successful pest management requires, early cypermethrin insecticide
resistance, such as RAPD-PCR techniques, for that H. armigera was collected from three hosts viz.,
cotton, chickpea, pigeonpea and screened across two cypermethrin resistance DNA specific marker
Le., GFOPAT05300 and GFABO4500, Overall, all population for GFOPATOSaoo marker showed 100 per
cent fidelity in all the three crops, and for GFABO4500 marker, cotton, pigeonpea showed and
chickpea showed 89.47, 100.0 and 90.9 per cent fidelity.
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