STUDIES ON GENETIC CHARACTERIZATION OF PERSIAN WALNUT (Juglans regia L.) GERMPLASM USING MOLECULAR MARKERS

Simple item page
dc.contributor.advisorKAUR, RAJINDER
dc.contributor.authorSHAILJA
dc.date.accessioned2018-03-22T08:05:20Z
dc.date.available2018-03-22T08:05:20Z
dc.date.issued2018-02
dc.description.abstractABSTRACTJuglans regia L . commonly known as ‘Akhrot’ and it belongs to family Juglandaceae.Walnut oil contains alphalinolenic acid and linoleic acid.They reduces the risk of cardiovascular diseases. In the present study EST-SSR markers were datamined for Juglans regia L.and were used for polymorphism studies. EST sequences of Juglans regia L.were downloaded from NCBI website (www.ncbi.nlm.nih.gov/nucest). 2000 EST sequences for Juglans regia L. were screened. ESTs containing SSR motifs were extracted out using an online tool, SSRIT (www.gramene.org/db/searches/SSRtool). SSR primers were designed from the EST-SSR containing sequences using PRIMER 3 software (www.frodo.wi-mit.edu/primer3/) and 98 primers were custom synthesized. Polymorphism study was carried out using 35 EST-SSR, 29 Genomic SSR and 30 ISSR primers among 37 genotypes of Juglans regia L. For polymorphism, DNA was isolated from young leaves of all the 37 genotypes using CTAB method (Doyle and Doyle, 1987). 24 primers were informative out of 35 EST-SSR, 19 primers were informative out of 29 Genomic SSR and 22 primers were informative out of 30 ISSR. Jaccard’s similarity matrix was developed and dendrograms were generated using NTSYSpc ver.2.02h to establish the percent similarity among the 37 genotypes of Juglans regia L. With EST-SSRs, two clusters ‘A’ and ‘B’ were obtained. Cluster A was further subdivided into A1 and A2. A1 contained 28 genotypes and A2 contained eight genotypes whereas cluster B contained only one genotype. For Genomic SSR two clusters ‘C’ and ‘D’ were obtained. Cluster C was further subdivided into C1 and C2. C1 contained 33 genotypes and C2 contained three genotypes whereas cluster D contained only one genotype whereas For ISSRs, 37 genotypes were divided into two cluster i.e ‘E’ and ‘F’. Cluster E was further subdivided into E1 and E2. E1 contained 28 genotypes and E2 contained eight genotypes whereas cluster F contained only one genotype. Morpho-physical characters were also studied for diversity study like Time of Bud burst, Flowering time, Degree of Dichogamy, Nut shape, Shell hardness, Nut weight, Kernel weight, Kernel recovery.Eucledia statistical tool is used to calculate the dissimilarity by combining both molecular as well as morphological data.Thus molecular markers and morpho-physical charaters used in the present study revealed a high level of polymorphism in the 37 genotypes of Juglans regia L., revealing their efficiency for diversity analysis studies.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810042363
dc.keywordsJuglans regia L,Akhrot,alphalinolenic acid and linoleic acid., cardiovascular diseasesen_US
dc.language.isoenen_US
dc.pages89+IIen_US
dc.publisherUHF,NAUNIen_US
dc.subAgricultural Microbiologyen_US
dc.subjectnullen_US
dc.themeJuglans regia L,Akhrot,alphalinolenic acid and linoleic acid., cardiovascular diseasesen_US
dc.these.typePh.Den_US
dc.titleSTUDIES ON GENETIC CHARACTERIZATION OF PERSIAN WALNUT (Juglans regia L.) GERMPLASM USING MOLECULAR MARKERSen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
PDF Ph.D. Thesis Shailja BTC (2018).pdf
Size:
4.47 MB
Format:
Adobe Portable Document Format
Description:
BIOTECHNOLOGY
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses