Bioassay for bruchid resistance and DNA fingerprinting in cowpea [Vigna unguiculata (L.) Walp.]
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Date
2008-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Bruchid (Calosobruchus chinensis L.) is an important store grain pest of pulses in general and cowpea [Vigna unguiculata (L.)Walp.] in particular. An experiment was conducted at Department of Genetics and Plant Breeding, Pantnagar to find out possible sources of resistance to bruchid and their biochemical and molecular analysis among ninety genotypes of cowpea which were obtained from IITA, Nigeria and Cowpea Research Centre, Pantnagar. Under initial screening we found thirty genotypes showing moderate to high level of resistance against bruchid. Again selected thirty genotypes were screened were screened following completely randomized design (CRD).Again genotypes were categorized showing moderate to high level of resistance to bruchid. Eighteen selected genotypes were biochemically screened for amylase inhibitor activity and it was found though variability exists for its activity but it may not be sole factor responsible for conferring resistance to bruchid beetle in case of cowpea. Finally selected ten genotypes were analyzed by RAPD (Random Amplified polymorphic DNA) for assessing genetic
variability existing among the selected genotypes. It was found that considerable variability exists among the cowpea genotypes. The findings of the above study will certainly aid in the identification of the donor sources of resistance to bruchid, in addition it also indicates possible involvement of other factors including or excluding the amylase inhibitor in conferring resistance to bruchid beetle.
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