SELECTION AND CHARACTERIZATION OF NAC TRANSCRIPTION FACTOR IN TRANSGENIC GROUNDNUT {Arachis hypogaea L.)
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Date
2007-09-17
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University of Agricultural Sciences GKVK, Banglore
Abstract
The ability of plants to tolerate drought conditions is crucial for agricultural production
worldwide. To meet the growing demand for food there is a need to increase crop resistance to
various abiotic stresses. One of the viable approaches for crop improvement towards abiotic
stress tolerance is by genetic engineering. Stress specific transcription factors bring about
coordinated expression of stress responsive genes involved in stress adaptation. ANAC102 is one
of the stress responsive gene belongs to NAC group of transcription factors. The transgenics
expressing NAC gene were earlier developed by in planta transformation method. In this
approach To plants chimeric and putative ANAC102 were identified in the Ti generation.
We had screened 96 Ti ANAC102 plants by Polymerized Chain Reaction. We got 30 positive
plants. These 30 plants were further characterized by two approaches. In the first approach T2
ANAC102 plants were screened by PCR .Amongst the 136 plants screened 50 were PGR
positives. Out of these 6 plants subjected to RT-PCR analysis these transgenic lines showed
higher ANAC102 transgene expression compared to wild type by using gene specific primers.
These plants have also shown high chlorophyll content and high cell viability compared to
control. The pod weight and pod yield was also high in transgenic plants compared to wild type.
Second approach is Antibiotic based intermediate screening of T2 ANAC102 groundnut
seedlings. Seedlings showing resistance to Kanamycin were sown under transgenic facility. Out
of 40 plants selected 36 plants found positive with gene specific primers and 30 plants found
positive with nptll primers. Selected plants observed for stress tolerance had shown higher
chlorophyll content, more cell viability, less membrane leakage and less water loss compared to
wild type plants. These promising lines should be analyzed further generation for the drought
tolerance.
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