CLONING OF 2-PHENYLETHANOL SYNTHESIZING GENE CYP79D73 FROM PLUMERIA RUBRA AND IN-SILICO ANALYSIS OF ITS ANTIMICROBIAL PROPERTIES AND GC-MS ANALYSIS OF AROMATIC COMPOUNDS
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Date
2023-02-24
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University of Agricultural Sciences, Bangalore
Abstract
Two-phenylethanol(2PE) is an organic compound that consists of a phenethyl group (C6H5CH2CH2) attached to OH. This metabolite is ubiquitously found in plants, but its highest expression has been noted in Frangipani (Plumeria rubra). This compound has antimicrobial, antiseptic and disinfectant property and is also known for its aromatic essence, hence used as a preservative in pharmaceutics and perfumery. Frangipani is well known for its brightly coloured and fragrant flowers and emits several floral volatile organic compounds (VOCs). This species has a flower-specific gene encoding a cytochrome P450 family 79D protein (PrCYP79D73), which is a crucial player in the biosynthesis of the major floral VOC- 2PE and other nitrogen-containing volatiles. In the present study, an attempt was made for isolation and cloning of 2PE encoding gene. The genomic DNA from frangipani was extracted and used for the amplification of 2PE encoding gene. An amplification product of ~1578 bp was obtained. An attempt was also made on analysing antiviral and antifungal properties of 2PE through molecular docking. The 2PE has showed affinity towards antiviral proteins such as Covid-19 protease, Hepatitis-B protein and HIV protease protein and affinity towards fungal proteins such as Aspergillus niger endoglucanase, Candida albicans myristoyl transferase and Rho1 protein of Penicillium chrysogenum indicating its potential application in making drugs. GC-MS analysis of aromatic compounds in Plumeria rubra in comparison with rose, champaca and Plumeria alba depicted compounds which are benzene derivatives and most of which are aromatic compounds and are widely used in perfumery industry, cosmetics preparation and pharmaceutical industry