GENETIC VARIABILITY FOR PHYTIC ACID IN FINGER MILLET (Eluesine coracana (L.) Gaertn.) GENOTYPES: MOLECULAR CHARACTERIZATION OF CONTRAST TYPES AND DEVELOPMENT OF Ipa MUTANTS
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Date
2008-08-28
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Micronutrients are required in small quantities but play a vital role in
various processes of human body. The intake of nutrients in daily diet is far from
satisfactory and largely less than 50 % RDA (recommended Daily Allowance) is
consumed by Indian population. Unavailability of the essential and vital
micronutrients in plant based food may be due to either low content of nutrients or
presence of anti-nutritional factor (ANF). One among the ANFs the phytic acid
presents in all cereals, pulses and oil seeds. Phytic acid chelates cations like Zn2+,
Fe2+, Ca2+ and Mn2+, makes them unavailable form. In this context made an
attempt to develop mutant for low phytic acid line in leading variety. Chemical
mutagen, 2 % (v/v) EMS was showed LD50 in GPU-45 variety. The mean phytic
acid content in M| seeds ranged from 190.2 to 210.7 mg per lOOg of seed. In wild
plants the mean phytic acid content was 202.1 mg per lOOg was observed. In this
study the 530 core germplasm lines of finger millet were assessed for their phytic
acid content in grains. There is a huge variation in phytic acid content and it
ranges from 60.2 to 517.7 mg per lOOg. The 20 contrasting genotypes for phytic
acid were analysed for zinc and iron content. Study showed distinct pattern of
correlation with phytic acid. Zinc showed high significant correlation with phytic
acid and high significant negative correlation was observed with phosphorus. Iron
was not correlated with the phytic acid and inorganic phosphorus. All the four
characters, zinc, iron, phytic acid and phosphorus are showed a good correlation
between GCV and PCV and all are highly heritable. The SSR two rice markers
RM 3542 and RM 482 were did not show polymorphism between low and high
phytic acid types of finger millet.
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