STUDIES ON AUGMENTATION CYSTOPLASTY USING ACELLULAR MATRIX OF SWIM BLADDER IN RABBITS
Loading...
Date
2012-12
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI – 517 502. (A.P) INDIA
Abstract
ABSTRACT:
The present study on augmentation cystoplasty was conducted in
rabbits to compare the suitability of auto grafts,fresh and acellular swim
bladder graft for its healing based on different parameters. . Fresh swim
bladders collected from fish were decellularized using 1M sodium chloride
solution and 0.5% Triton X-100 under gentle agitation which effectively
removed the cellular material from the tissue and was confirmed by
Haematoxylin & Eosin .Histologically swim bladder contains an outer
fibromuscular layer, muscularis mucosae below the submucosa and internal
layer of cuboidal secretory epithelium A total of 24 rabbits of either sex divided in to three groups were used
for urinary bladder wall reconstruction with auto grafts,fresh and acellular
swim bladder graft after creating a defect . Glycopyrolate as premedicant
and Xylazine – Ketamine mixture provided smooth induction and sufficient
plane of anaesthesia in rabbits. Parenteral administration of enrofloxacin and
daily dressing of the wound with 5% povidone iodine minimized the
complications of wound healing. There was a significant rise (P < 0.05) in the rectal temperature,
respiratory and heart rate values in the initial postoperative days in all the
animals . A significant decrease (P < 0.05) in haemoglobin and PCV in the
initial postoperative days in all the animals.Significant increase (P < 0.05) in
creatinine levels at 14 th day in groups I and III while in group II there was a
nonsignificant rise on 3rd and 5 th day followed by a significant increase up to
day 14. There was a significant increase (P < 0.05) in urea levels at 1 st day
in all the groups and a non significant increase in levels upto 13 th post 3rd,7th and 14 th day in all the groups .
Cystograms showed inconsistent augmentation effect in auto and fresh
swim bladder graft animals while it was considerable with acellular graft
group. The cutaneous wound healed by 12-15 days.Grossly, grafted site
showed various forms of adhesions in all the animals, The graft showed
intactness amd as time progresses . Histologically all the sections showed
inflammatory cells at the host graft interface at the early phase of observation
and as time progresses marked fibroblastic activity formation of thin fibrous
tissue and neocapillaries are the findings in acellular graft group. In
conclusion the acelluar swim bladder was well accepted by the host tissue and
is ideal for augmentation cystoplasty rabbits followed by auto graft and fresh
swim bladder graft. .Because of early degradation of scaffold with deposition
of native tissue, proliferation of epithelium, along with high degree of
angiogenesis and lesser inflammatory reaction, acellular swim bladder graft
can be graded superior to autograft and the fresh swim bladder grafts.
Description
THESES
Keywords
null