STUDIES ON IN VITRO PROPAGATION OF BLUEBERRY(VACCINIUM CORYMBOSUM L.) - A MEDICINALLY IMPORTANT FRUIT SPECIES
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Date
2013
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ABSTRACT
A protocol for in vitro propagation was developed for Vaccinium corymbosum, a medicinally important fruit species. The sterilized explants (buds) cultured on MS and WPM supplemented with 5.0 mg/l 2-iP + 1.0 mg/l GA3 and 2.5 mg/l zeatin + 1.0 mg/l GA3, respectively gave best results for in vitro shoot bud establishment. Out of the two media found best for bud establishment, WPM supplemented with 2.5 mg/l zeatin + 1.0 mg/l GA3 proved to be the best for in vitro shoot multiplication. Also comparison of different growth regulators viz., zeatin, 2-iP and BAP in both MS as well as WPM was studied and out of these three different growth regulators, zeatin was found to be best for in vitro shoot multiplication. Leaves were found to be the best explants for callus induction from both in vivo and in vitro explants on both MS as well as WPM supplemented with 0.25 mg/l TDZ + 0.05 mg/l IBA in each case. The callus obtained with both in vivo and in vitro leaf explants was compact nodular soft in texture and green in color. For shoot regeneration from callus, it was found that in vivo shoot segments derived callus when cultured on WPM medium supplemented with 2.0 mg/l zeatin showed best results. Root primordium formation was observed on basal WPM supplemented with different concentrations of IBA viz., 1.0 mg/l and 2.0 mg/l and also with 0.2 % activated charcoal. Ex vitro rooting was carried out and 80.00% of survival rate was obtained on rooting mixture containing cocopeat : perlite (1:1).