Morpho-physiological, biochemical and protein profiling under drought stress in selected chickpea (Cicer arietinum L.) varieties
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Date
2022-07
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CCSHAU Hisar
Abstract
Chickpea (Cicer arieitnum L.) is an important food legume crop of the family Fabaceae with high protein levels that is widely grown in rainfed areas prone to drought stress. It is a self-pollinated cool season crop with true diploid (2n=16) nature. They are relatively cheaper and higher source of protein. About 90% chickpea crop is grown in rainfed conditions without depending on irrigation. In the present study, two varieties of chickpea namely ICC 4958 and HC-6 were grown under 3 field capacities (100% FC, 50% FC and 25% FC). Sampling was taken 3 times i.e., 15, 30 and 45 days after sowing (DAS). Parameters like morphological, physiological, biochemical, metabolite and protein profiling of the two varieties was done.
Under morphological parameters, shoot length, number of branches number of leaves, number of flowers declined as the drought level increased. Under physiological parameters, relative water content (RWC), cell membrane stability (CMS), chlorophyll content decreased in both the varieties as field capacity decreased. Under biochemical parameters, proline, sugar, glycine betaine, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) increased as drought stress increased. Under metabolite analysis, MDA (Malondialdehyde) content, total anthocyanin, flavonoid content, phenolic content increased as drought progressed. We also performed protein profiling of the two varieties using SDS-PAGE (Sodium dodecyl-sulfate polyacrylamide gel electrophoresis) to differentiate the expression analysis of the two varieties.