MICROSATELLITE MARKER BASED ASSESSMENT OF GENETIC STRUCTURE OF MACHERLA BROWN SHEEP
| dc.contributor.advisor | Punya kumari, B (Major) | |
| dc.contributor.advisor | Jaya laxmi, P | |
| dc.contributor.advisor | Suresh, J | |
| dc.contributor.author | MOHAN KISHORE, MOLABANTI | |
| dc.date.accessioned | 2016-12-15T17:10:54Z | |
| dc.date.available | 2016-12-15T17:10:54Z | |
| dc.date.issued | 2015-12 | |
| dc.description.abstract | ABSTRACT: Macherla Brown sheep is the native sheep distributed along the banks of river Krishna flowing through Guntur, Krishna, Prakasam and Nalgonda districts of Andhra Pradesh and Telangana states. The home tract is mainly from the Nagarjuna sagar dam areas of Guntur and Nalgonda. Macherla Brown is known for heat tolerance, disease resistance and thrives well in harsh climatic conditions and scarce feeding conditions. The assessment of genetic variation is important to characterize the populations for breed recognition, conservation and also to determine the level of inbreeding in the population. The present study was carried out to assess the genetic structure of Macherla Brown sheep at molecular level to understand the genetic variability and diversity of population and to recognise this high performing local variety of sheep as a breed. Twenty four ovine microsatellite markers recommended by ISAG/FAO for population diversity studies in sheep were used in the present study. Phenol-chloroform method was used for the isolation of DNA from the collected blood samples and the microsatellite markers were amplified by PCR reaction. The amplicons were identified using agarose gel electrophoresis. The genotypes were scored using regression equation of the 100 bp DNA standard. The number, size and frequency of alleles were calculated using POPGENE version 1.3.2 software. A total of 275 alleles were observed for the twenty four marker loci under investigation in the sampled Macherla Brown population. The number of alleles at each locus varied from a minimum of eight (BM6506, HSC, OarCP34) to a maximum of eighteen (MAF214) with a mean of 11.458 alleles. Allele size ranged from a minimum of 69 bp (OarCP20) to a maximum of 313 bp (MAF214), while allele frequency varied between 0.0102 (HSC, INRA63, MAF214, OarP49 and OarVH72) to 0.2551 (HSC). The number of effective alleles across twenty four microsatellite loci studied ranged from 5.8065 (HSC) to 14.1652 (OarFCB48). The mean number of effective alleles was 9.597 ± 2.454. The observed heterozygosity ranged from 0.0612 (BM8125) to 0.2653 (OarCP34) with a mean value of 0.110 ± 0.049 whereas, the expected heterozygosity ranged from 0.8278 (HSC) to 0.9294 (OarFCB48) with a mean value of 0.889 ± 0.029. All the twenty four microsatellite loci (100 percent) were found to be highly polymorphic and the PIC values ranged from 0.8052 (HSC) to 0.9295 (OarFCB48). The mean PIC value for all the twenty four loci was found to be 0.882 ± 0.036. The Chisquare test revealed that all the twenty four loci were showing significant deviation from Hardy-Weinberg Equilibrium. The inbreeding estimates obtained in this study were all positive and ranged from 0.6915 (OarCP34) to 0.9341 (OarFCB48) with the mean FIS value of 0.876 ± 0.057 indicating the high deficiency of heterozygotes. | en_US |
| dc.description.sponsorship | DEPARTMENT OF ANIMAL GENETICS AND BREEDING, COLLEGE OF VETERINARY SCIENCE, SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI – 517502. (A.P.) INDIA | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/90363 | |
| dc.language.iso | en | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY, TIRUPATI – 517502. (A.P.) INDIA | en_US |
| dc.sub | Animal Genetics and Breeding | en_US |
| dc.subject | Macherla Brown; sheep; GENETIC STRUCTURE; microsatellite markers | en_US |
| dc.these.type | M.Sc | |
| dc.title | MICROSATELLITE MARKER BASED ASSESSMENT OF GENETIC STRUCTURE OF MACHERLA BROWN SHEEP | en_US |
| dc.title.alternative | MVSc;CVSc; TPTY; AcC No: T1724 | en_US |
| dc.type | Thesis | en_US |