ISSR Markers Based Genetic Diversity Analysis in Kiwifruit (Actinidia deliciosa (A.Chev.) CF Liang et AR Ferguson) Genotypes
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Date
2019-09
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CCSHAU, Hisar
Abstract
Kiwifruit (Actinidia deliciosa (A. Chev.) CF Liang et AR Ferguson) (2n=6x=174) also known as Chinese goose berry and the horticultural wonder of New Zealand, is a commercially important crop and a very good source of Vitamin C. The present study was undertaken for assessing genetic diversity in Kiwifruit genotypes using ISSR primers and for identification of any probable ISSR marker linked to sex in Kiwifruit genotypes. A total of seven genotypes of Kiwifruit were used that included two male (Tomuri and Allison (M)) genotypes and five female genotypes (Allison, Abbott, Bruno, Hayward, Monty). Genomic DNA was isolated from young leaves of Kiwifruit genotypes. For molecular analysis 104 ISSR primers were screened, out of which 24 ISSR primers amplified and were polymorphic. These were employed for genetic diversity analysis and identification of genotype. Among these polymorphic ISSRs, eight ISSRs were found to identify selected genotypes on the basis of unique amplicons. IS7, IS11, IS93, IS88, IS114, IS107, IS101 and IS79 could identify genotypes . One SSR marker, A 003 reported earlier was also validated. It could identify Allison (M). Similarity matrices of seven kiwifruit genotypes revealed that similarity value among different genotypes ranged from 0.5298 to 0.7094. A maximum similarity value of 0.7094 was observed between Allison (M) and Tomuri whereas Abott and Allison (F) were found to be most diverse. Molecular analysis showed that Allison (M) and Tomuri were much similar. Abbott was found most diverse when compared to other kiwifruit genotypes. The results of present study can be utilized for sex and genotype identification at seedling stage and in kiwifruit improvement programme.