Biochemical characterization and molecular mapping of stripe rust resistance gene(s) in barley (Hordeum vulgare L.)

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dc.contributor.advisorBhardwaj, Rachana D.
dc.contributor.authorSingla, Prabhjot
dc.date.accessioned2020-07-28T07:42:32Z
dc.date.available2020-07-28T07:42:32Z
dc.date.issued2020
dc.description.abstractThe study was conducted to appraise the mechanisms of stripe rust resistance in four barley genotypes including three resistant (RD2900, RD2901 and RD2552) and one susceptible (Jyoti) genotypes against different races of Puccinia striiformis f. sp. hordei. Disease reaction showed RD2901 as immune to very resistant followed by RD2552 as immune to resistant and RD2900 as moderately resistant and Jyoti as susceptible against M, G, 57, Q and 24 races. RD2901 possessed higher antioxidant potential in terms of free radical scavenging activities and phenolic compounds under control conditions and maintained sufficiently high on inoculation with different races. Upregulation of NOX, POX, CAT, APX, MDHAR, DHAR, GR, PR proteins, PAL, TAL, GDH and OAT with a concomitant decrease of H2O2 and MDA in RD2901 could also play a vital role in resistance against stripe rust. It also showed maintained levels of β-glucan and increased contents of lignin, proline, glycine betaine and choline. GSH/GSSG significantly declined in Jyoti and increased or remained unaffected in resistant genotypes that might be playing a key role in imparting tolerance. 2-DE showed 36 DEPs in RD2901 under control and infected conditions. Among them, 14 proteins were over expressed and 12 proteins as newly synthesized under infected conditions. Inheritance studies showed that F2 population (RD2901  Jyoti) segregated into 31 resistant and 89 susceptible individuals that fit well to 1:3 which is expected of single recessive resistant gene. BSA indicated that Bmag0500 marker located on 6H chromosome of barley genome was found to be linked with resistant gene.en_US
dc.identifier.citationSingla, Prabhjot (2020). Biochemical characterization and molecular mapping of stripe rust resistance gene(s) in barley (Hordeum vulgare L.) (Unpublished Ph.D. Dissertation). Punjab Agricultural University, Ludhiana, Punjab, India.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810149952
dc.keywords2-DE, BSA, defence system, Hordeum vulgare L., redox buffer, stripe rusten_US
dc.language.isoenen_US
dc.pages131en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemBiochemical characterization and molecular mapping of stripe rust resistance gene(s) in barley (Hordeum vulgare L.)en_US
dc.subBiochemistryen_US
dc.subjectnullen_US
dc.themeBiochemical characterization and molecular mapping of stripe rust resistance gene(s) in barley (Hordeum vulgare L.)en_US
dc.these.typePh.Den_US
dc.titleBiochemical characterization and molecular mapping of stripe rust resistance gene(s) in barley (Hordeum vulgare L.)en_US
dc.typeThesisen_US
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