DUS CHARACTERIZATION AND DIVERSITY ANALYSIS OF INBRED LINES IN PEARL MILLET (Pennisetum glaucum (L.) R. Br.)
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Date
2024-04-24
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Acharya N G Ranga Agricultural University
Abstract
The present investigation was conducted at Agricultural Research
Station (ARS), Perumallapalle, Tirupati during rabi, 2021 to characterize
pearl millet inbred lines for DUS traits and to identify potential germplasm
lines by estimating genetic parameters (variability, heritability and genetic
advance), genetic divergence, character association and path coefficient.
DUS characterization of 70 pearl millet genotypes using 28 DUS traits
revealed existence of abundant diversity for these characters. In the present
studied pearl millet inbred lines appreciable differences were observed for the
traits viz., anthocyanin pigmentation, leaf sheath length, leaf blade length, leaf
blade width, spike stigma pigmentation, anther colour, plant node pubescence,
number of nodes plant-1, node pigmentation, internode pigmentation, spike
length, anthocyanin pigmentation of glume, spike bristle, spike bristle colour,
bristle appearance, spike girth, number of productive tillers plant-1, plant
height, spike shape, spike-density, seed color, seed shape and 1000 seed
weight. Based on the DUS descriptors inbred lines were characterized
effectively which would be useful for their documentation and registration.
These descriptors would aid in explicit identity of inbred lines and help in
maintenance of their purity in field for use in future breeding programmes.
The analysis of variance carried out among 70 germplasm lines for 21
yield and yield attributes revealed significant differences for all the characters
indicating the presence of considerable amount of genetic variability for the
characters in the studied material.
The characters such as grain yield plant-1, 1000 grain weight panicle
weight, green fodder yield plant-1, dry fodder yield plant-1 and harvest index
showed higher estimates of PCV and GCV indicating ample amount of
variation among germplasm lines for these traits. Thus, direct selection for
these traits would result in further improvement of grain yield. High
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heritability coupled with high genetic advance as per cent of mean was
observed for days to 50 % flowering, spike length, spike girth, number of
productive tillers plant-1, plant height, 1000 grain, panicle weight, green
fodder yield plant-1, dry fodder yield plant-1, threshing percentage, harvest
index and grain yield plant-1 indicating the predominance of additive gene
action and direct selection would be effective for improvement of these traits.
D2
analysis grouped 70 inbred lines into 12 clusters. Among all the
characters studied, 1000 grain weight, days to 50% flowering and number of
productive tillers plant-1 contributed relatively maximum towards the total
genetic divergence. Inter cluster distance was observed maximum between
cluster VI and XII followed by cluster X and XII, cluster III and XII and
cluster IV and XII representing that germplasm lines belonging to these
clusters are more divergent.
Correlation studies revealed that characters viz., panicle weight
followed by green fodder yield plant-1, dry fodder yield plant-1, number of
productive tillers plant-1, spike girth, plant height, leaf blade length, number of
nodes plant-1, 1000 grain weight, spike length, harvest index, threshing
percentage, leaf blade width and specific leaf area at 45 DAS had significant
positive association with grain yield plant-1 indicating simultaneous selection
of these traits would result in improvement of grain yield.
Further, path analysis estimates in the present investigation revealed
that panicle weight followed by threshing percentage, green fodder yield
plant-1 had true relationship with grain yield plant-1 by establishing significant
positive association and high positive direct effect on grain yield plant-1. Low
residual effects at both phenotypic and genotypic level demonstrated that
choice of traits in the present study were able to explain most of the effects on
grain yield.
Superior inbred lines PPBi-3, PPBi-46, PPBi-47, PPBi-50 and PPBi-59
identified in the present study could be utilized for the development of
composites.