TAGGING AND MAPPING OF MUNGBEAN YELLOW MOSAIC VIRUS (MYMV) RESISTANCE LOCI AND MOLECULAR CHARACTERIZATION OF MYMV ISOLATE IN MUNGBEAN [Vigna radiata (L.) WILCZEK]
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Date
2018-11-03
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Mungbean (Vigna radiata (L.) Wilczek) subgenus Ceratotropis, is a fast-growing
warm-season legume, native to the Indian subcontinent. It is an important pulse crop
because of its short growth duration, low water requirement, and as a crop suitable for
rotation and nutritional security being a rich source of protein. Biotic stress produced by
viruses especially mungbean yellow mosaic virus (MYMV) is the main constraint in its
production causing substantial yield losses. In the present investigation molecular
characterization of yellow mosaic virus (YMV) was done by using the coat protein (CP)
gene of five isolates collected from Dharwad, Belgaum, Amravati, Gulbarga and
Bengaluru to confirm their identity; isolates from Dharwad and Belgaum were classified
as mungbean yellow mosaic India virus (MYMIV) and isolates from Amravati, Gulbarga
and Bengaluru as MYMV. The phylogenetic analysis revealed predominance of MYMIV
in Nothern, Eastern and Central India while MYMV predominates in Southern India, this
could help in developing a transgenic strategy for managing this disease. One hundred
and ninety two mungbean genotypes were field screened and parents PS16 (Susceptible)
x IC-39293 (Resistant) were further confirmed for disease incidence in greenhouse
experiment. A genetic linkage map was constructed for mungbean with a minimum LOD
of 2.5 and maximum recombination fraction of 0.3 threshold value from F2 population
developed from parents (PS16 x IC-39293) by using thirty one polymorphic SSR
markers, which mapped the DNA into four linkage groups (LGs) spanning a total length
of 211.64 cM with an average marker density of 6.82cM. Half the number (50 %) of
markers (14) mapped to LG 1. The number of markers mapped per linkage group varied
from 14 (LG 1) (highest marker density) to 3 (LG 3) (least marker density). These two
strategies singly or in combination could help in developing mungbean with resistance to
MYMV.