:Studies on epidemiology and host resistance of lentil wilt
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Date
2021-09-25
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Palampur
Abstract
Wilt of lentil, caused by Fusarium oxysporum f. sp. lentis is a serious disease caused by
Fusarium oxysporum f. sp. lentis and plays a major role in reducing lentil yield in India and
world. The present studies entitled “Studies on epidemiology and host resistance of lentil
wilt” was undertaken to study the factors affecting pathogen and disease development and to
evaluate the lentil germplasm for resistance against wilt disease. Isolation of the pathogen was
done from the diseased samples and pathogenicity was proved. F. oxysporum f. sp. lentis was
ascertained on the basis of symptomatological and morpho-cultural characteristics. The best
media for the growth of the pathogen were PDA (Potato dextrose agar) and MEA (Malt
extract agar) and the optimum temperature was ≥25OC early aged cultures were more virulent
and gave maximum (88.26 per cent) disease incidence. It was observed that disease incidence
increased with decrease in soil moisture level and vice versa. The disease incidence was
maximum (10.74 per cent) in early sown crop as compared to the late sown crop, however the
yield was observed maximum at the second date of sowing i.e. 15th November. Temperature
was positively correlated, and relative humidity was negatively correlated with the disease
incidence. Coefficient of determination revealed that the temperature, relative humidity and
rainfall contributed 96.7, 94.4, 96.0 and 98.0 per cent towards the disease incidence of wilt on
crop sown on 15th October, 30th October, 15th November and 30th November, respectively.
AUDPC also followed the same trend of disease incidence at different dates of sowing.
Genotypes EC-223235, IC-212688, IC-231785 and IC-240910 with disease incidence of <1
per cent were highly resistant whereas, genotypes IPL 316, IPL 526, IC-241260, LL 1641 and
1698 with disease incidence of ≤2 per cent were resistant under field conditions. Genotypes
LL 1641, EC-223235, IC-240910, IC-231785, IC-241260 and LL 1641 were highly resistant
under field and pot culture conditions.