Association of COL1A2 (PvuII) gene polymorphism with risk and severity of dental fluorosis – A case control study
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Date
2019
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Abstract
Dental fluorosis is a foremost public health problem in many countries,
including India. Very few studies investigated gene polymorphism and risk of dental fluorosis.
Genetic polymorphisms in Collagen Type I, alpha 2 (COL1A2) gene, found to be linked with bone
pathogenesis, may affect the tooth formation resulting in the vulnerability to dental fluorosis.
Aim: To assess the association between COL1A2 (PvuII) gene polymorphism and risk as well as
severity of dental fluorosis.
Methods: The present case control study was conducted among participants with (n = 60) and
without (n = 60) dental fluorosis. Dental fluorosis was assessed using Modified Dean’s fluorosis
index (1942). The PvuII polymorphisms (in exon 25) inside the COL1A2 gene were genotyped by
Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) procedure.
Statistical analysis were carried out with Chi-square test and Odds Ratio (OR) was determined with
multivariate logistic regression analysis.
Results: The genetic polymorphism in COL1A2 PvuII was found to be associated with the risk
of dental fluorosis which was highly significant (p< 0.001). The odds ratio was 31.4 times
[OR = 31.9, 95% CI: 3.9–48.7] higher for the homozygous PP genotype group and 4.0 times
[OR = 4.0, 95% CI: 1.0–10.7] higher for the heterozygous Pp genotype.
Description
TNV_SDJ_2019_31(463-468)
Keywords
Veterinary Science