MOLECULAR CHARACTERIZATION OF NELLORE SHEEP USING MICROSATELLITES
| dc.contributor.advisor | Punya Kumari, B (Major) | |
| dc.contributor.advisor | Gnana Prakash | |
| dc.contributor.advisor | Rao, V H | |
| dc.contributor.author | VANI, S | |
| dc.date.accessioned | 2017-06-10T13:11:41Z | |
| dc.date.available | 2017-06-10T13:11:41Z | |
| dc.date.issued | 2012-12 | |
| dc.description.abstract | ABSTRACT: Nellore, tallest breed of indigenous sheep is the predominant breed present in Andhra Pradesh. The animals true to the breed are mainly found in Nellore and Prakasam districts of Andhra Pradesh. Nellore is a meat type breed known for heat tolerance, disease resistance and thrives well in harsh conditions. To form the basis for evolving further strategies to improve animal production, the assessment of the genetic and production potential of our livestock is very much necessary. The present study was carried out to characterise Nellore sheep at molecular level to understand the genetic constitution of population which will be helpful to breeders to formulate breeding strategy for the improvement of the breed. The characterisation was done by ten microsatellite markers recommended by FAO for population diversity studies in sheep. DNA was isolated from blood samples by phenol-chloroform method and microsatellites were amplified by PCR reaction. The PCR products were resolved using agarose gel electrophorosis. The genotypes were scored and the number, size, frequency of alleles were calculated using POPGENE software. In the sampled population for the ten marker loci under investigation a total of 75 alleles were observed. The number of alleles at each locus varied from a minimum of four to a maximum of ten with a mean of 7.5 alleles across all loci. Of the 75 alleles observed, four alleles at loci OarVH72, seven alleles at BM1314, BM6526, OarFCB48 and OarHH41were found, whereas, eight alleles were detected at loci BM8125, CSSM47. The number of alleles at loci BM757 were nine and ten at OarFCB128. The most frequent allele number was seven. Of the ten loci studied, the effective number of alleles ranged from 3.2125 (OarVH72) to 5.7645 (OarCP34) with the mean number of 4.7202 across all loci. PIC values for Nellore sheep ranged from 0.637 (CSSM47) to 0.8157 (OarCP34) with mean PIC value of 0.7528. Based on the PIC values, it could be inferred that all the markers used in the present study showed values more than 0.5 indicating their importance in molecular characterization and genetic diversity studies. All the loci under investigation showed significant Chi-Square values, suggesting departure from Hardy-Weinberg Equilibrium proportions. The observed heterozygosity values varied from 0.2368 (OarVH72) to 0.833 (BM8125) with a mean value of 0.497 and the expected heterozygosity values varied from 0.6887 (OarVH72) to 0.8265 (OarCP34) with a mean value of 0.7865. Two out of ten loci revealed negative FIS indicating an absence of inbreeding in those loci while the remaining loci revealed positive FIS values ranging from 0.0831 (OarFCB128) to 0.4224 (OarCP34). The mean FIS value observed over all the loci was 0.1889 indicating the deficiency of heterozygotes. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810019290 | |
| dc.keywords | NELLORE SHEEP; MOLECULAR CHARACTERIZATION; MICROSATELLITES | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 101 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY , TIRUPATI – 517502. (A.P.) INDIA | en_US |
| dc.sub | Animal Genetics and Breeding | en_US |
| dc.these.type | M.V.Sc. | |
| dc.title | MOLECULAR CHARACTERIZATION OF NELLORE SHEEP USING MICROSATELLITES | en_US |
| dc.type | Thesis | en_US |