Identification of QTLs linked to early maturity and yield-related traits in horsegram (Macrotyloma uniflorum).

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dc.contributor.advisorChahota, R.K.
dc.contributor.authorKatoch, Megha
dc.date.accessioned2020-01-24T05:36:35Z
dc.date.available2020-01-24T05:36:35Z
dc.date.issued2019-07-23
dc.description.abstractMacrotyloma uniflorum is an important, self pollinated diploid (2n=2x=20) food legume with probable genome size of 400Mbps. Limited genomic resources and lack of genetic variation are major constrains in its genetic improvement. Further, horsegram production is hampered due to twining growth habit, longer days to maturity, photosensitivity and indeterminate growth habit. The present study was aimed to construct linkage map of an intraspecific F8 RILs population of 162 individuals derived from HPKM249×HPK4 of horsegram and identification of genomic regions linked to early maturity and yield related traits. Two thousand and eleven molecular markers were screened for parental polymorphism and 493 (25.42 %) were found to be polymorphic among the parents. Of these, 295 were mapped on ten linkage groups at LOD 3.5 spanning 1541.7 cM with an average marker density of 5.20 cM. Analysis of variance of 162 RILs revealed significant differences for all the measured traits. Phenotypic data from the RILs were used to identify QTLs for early maturity and yield related traits by composite interval mapping (CIM). A total of 27 QTLs (LOD ≥ 2.5) were detected across the three environments (Palampur 2016, Palampur 2017, Bajaura 2017) and combined data) for 24 traits. Among these, 15 were major QTLs with PVE greater than ten per cent and five were stable QTLs across locations and years. Phenotypic variation explained (PVE) by QTLs ranged from 6.4 to 53.4 per cent. The highest phenotypic variation (53.4 %) was explained by the QTLs for root length. In conclusion, it is envisaged that the present linkage map, fortified with 295 SSR markers and 27 QTLs for early maturity and yield-related traits would provide genomics tools to breeders for further genetic enhancement of this crop species. Thus, the current study will serve as a strong foundation for further validation and fine mapping of QTLs for utilization in horsegram breeding programs.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810141690
dc.keywordsHorsegramen_US
dc.language.isoenen_US
dc.pages170en_US
dc.publisherCSK HPKV, Palampuren_US
dc.subAgricultural Biotechnologyen_US
dc.subjectnullen_US
dc.themeTo construct linkage map of Macrotyloma uniflorum using morphological and DNA markers.en_US
dc.these.typePh.Den_US
dc.titleIdentification of QTLs linked to early maturity and yield-related traits in horsegram (Macrotyloma uniflorum).en_US
dc.typeThesisen_US
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