RELEVANCE OF STARCH HYDROLYSIS AS AFFECTED BY 1-DNJ, AN ALPHA-GLUCOSIDASE INHIBITOR IN SEEDS AND MESOPHYLL TISSUES OF MULBERRY
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Date
2022-12-18
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Abstract
Mulberry is often used as medicine in Chinese and Indian cultures, as it is known to contain many bioactive compounds. One such unique metabolite is 1-Deoxynojirimycin (1- DNJ), an iminosugar analogue to D-Glucose, making it a potent α-glucosidase inhibitor. This property has led 1-DNJ to be used in therapeutics and has been studied in mammalian systems. Present research outlines the investigation of 1-DNJ and its role in plant species. 1-DNJ being a known competitive inhibitor of α-glucosidase, its role in starch hydrolysis during seed germination and leaf starch hydrolysis at night time is hypothesized. This study reveals that the seed germination and seedling vigour of mulberry, rice and wheat seeds are affected in the presence of 1-DNJ with highest inhibitions of germination noticed in mulberry seeds and least inhibition in paddy seeds. However, starch hydrolysis at night in leaf is not affected, as 1-DNJ
levels in leaf extract of mulberry are diurnally regulated. Studies on starch content and maltase enzyme (target of 1-DNJ) activity highlights that the starch content in DNJ or SA treated leaf gets reduced and maltase enzyme activity to be increased during night time similar to untreated control. Further, metabolites such as glucose, fructose and sucrose are increased in night in DNJ or SA treated leaves as in untreated leaves, in line with starch hydrolysis. Thus, the diurnal regulation of 1-DNJ content and its role in starch metabolism in mulberry is being reported for the first time. The similar study in contrasting genotypes would strengthen the present findings.
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