COEXPRESSION OF THREE NUCLEAR FACTOR (NF) GENES IN RICE TO IMPROVE ABIOTIC STRESS TOLERANCE
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Date
2013-07-29
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University of Agricultural Sciences, GKVK
Abstract
Plants evolved multiple adaptive strategies to survive in the ever changing environment.
Adaptive responses are associated with altered expression of stress responsive upstream
regulatory genes such as transcription factors (TFs) and downstream regulatory genes. In
addition to stress specific genes activity of housekeeping regulatory genes such as TFs play a
vital role in adaptive response. It has been reported that Nuclear Factor Y (NF-Y), a histone like
CCAAT binding protein composed of NF-YA, NF-YB and NF-YC has a role in imparting stress
tolerance. From this context, the major objective of this study was to examine the relevance of
simultaneous expression of subunits of NFYs in imparting tolerance to abiotic stress in rice.
Rice transgenic plants harboring igrA, NF-YA3, NF-YB1 and NF-YC2 were developed by
Agrobacterium mediated in planta transformation technique using rice genotype JBT36/14. In
this study, T1 lines were advanced to T2 and T3 generations. In each generation, the putative
transgenic lines were evaluated by various physiological and molecular techniques. The
transgenic plants co-expressing all the three NF-Y subunits, performed significantly better than
the wild type under PEG8000-, NaCl-, CdNO3- and Methyl Viologen- induced stresses. The
viability of the pollen was monitored in osmotically stressed tillers. The transgenic plant had
significantly higher pollen viability compared to wild type. The transgenic plants showed better
shoot and root phenotype compared to wild type. The study revealed that the co-expression of
NFY subunit can impart tolerance to different abiotic stresses in rice.
Key words: Trancription factors, NFYs, coexpression, abiotic stress.
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