Candidate Gene Mapping, Pyramiding of Stay-Green and Water use Efficiency Qtls and Mapping of Putative Qtls for Root Traits using Ril Populations in Sorghum
| dc.contributor.advisor | B. Fakrudin | |
| dc.contributor.author | Rajkumar | |
| dc.date.accessioned | 2016-07-25T13:57:47Z | |
| dc.date.available | 2016-07-25T13:57:47Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | Recombinant Inbred Populations (RIPs) derived from the crosses IS9830 x E36-1 (RIP1) and N13 x E36-1 (RIP2) were used to map localize the drought tolerance-related candidate genes through SNPs. A total of five QTLs flanked by at least one candidate gene (SNP) were found common across both RIPs: qGLA3 (Xtxp38-Xsnp19); qGLA4 (Xiabt227-Xsnp56) qDAF2 (Xiumc136-Xsnp28) qPL4 (Xisp229-Xsnp36) and qSY4 (Xsnp36-Xtxp27). The three stable QTLs for stay-green, qSTG1A:Xtxp34-Xtxp285; qSTG2: Xtxp205-Xtxp231; qSTG3:Xtxp298-Xtxp324 and one QTL for water use efficiency, qCID2:Xtxp6-Xiabt70 were introgressed from E36-1 the stay-green parent into SPV86, SPV570 and M35-1. The foreground and limited background screening across BC1F1, BC2F1, and BC3F1 generations was done and the BC3F2 progenies with various QTL combinations in homozygous condition were identified and selfed. The BC3F3 near isogenic lines (NIL) were evaluated in greenhouse: NIL with all four QTLs in M35-1 (75.83 %), SPV86 (78.48 %) and SPV570 (75.57 %) backgrounds showed per cent green leaf area (GLA) retention on par to the stay-green donor parent (E36-1, 75.20 %) at physiological maturity. The two RIPs: E36-1 x Basavanapada (RRIP1) and E36-1 x SPV70 (RRIP2) segregating for high root volume and root length respectively were field evaluated during rabi 2010-11. Analysis of variance revealed significance difference among the RILs. A total of 113 (55/530 EST-SSRs, 40/275 SSRs, 18/138 SNPs) and 104 (50/530 EST-SSRs, 34/270 SSRs, 20/138 SNPs) polymorphic markers were genotyped to construct a genetic linkage map spanning 1633 cM and 1800.1 cM, and a total of 30 and 28 putative QTLs were mapped for root and yield related traits in RRIP1 and RRIP2, respectively. The lack of overlap between the QTLs of component traits of root and yield, suggested that these two parameters are independent and the possibility of combining these two traits, favorably to enhance productivity of sorghum under receding moisture condition. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/69776 | |
| dc.publisher | UAS Dharwad | en_US |
| dc.sub | Plant Biotechnology | |
| dc.these.type | Ph.D | |
| dc.title | Candidate Gene Mapping, Pyramiding of Stay-Green and Water use Efficiency Qtls and Mapping of Putative Qtls for Root Traits using Ril Populations in Sorghum | en_US |
| dc.type | Thesis | en_US |