POTENTIAL OF ANTAGONISTIC YEASTS, BOTANICALS AND GRAS CHEMICALS AGAINST POST-HARVEST ROT CAUSED BY Penicillium digitatum Sacc. IN KINNOW MANDARIN

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dc.contributor.advisorGAUTAM, HARENDER RAJ
dc.contributor.authorARAVINTHKUMAR A
dc.date.accessioned2022-11-26T10:44:09Z
dc.date.available2022-11-26T10:44:09Z
dc.date.issued2022-10
dc.description.abstractABSTRACT The present investigation titled “Potential of antagonistic yeasts, botanicals and GRAS chemicals against post-harvest rot caused by Penicillium digitatum Sacc. in Kinnow mandarin” was conducted in the laboratories of the Department of Plant Pathology, Dr. YS Parmar University of Horticulture and Forestry, Nauni-Solan. During the survey in different Kinnow growing areas of North India (2021-2022), disease incidence of the post-harvest green mould rot ranged between 14 and 27.5 per cent. Maximum severity (23%) of disease was recorded in Kinnow wholesale market of Abohar. Seven yeast isolates isolated from different sources, nine locally available botanicals and six different GRAS chemicals were evaluated against Penicillium digitatum under in vitro conditions. Among yeasts, Saccharomyces cerevisiae strains viz., R, BK 4 and BK 6 were found to inhibit the mycelial growth of the pathogen by 66.66, 53.50 and 48.14 per cent, respectively. Among botanicals, aqueous extracts of Ashy Roylea (84.81%), Curry leaf (78.70%) and Peppermint (76.47%) were found to be effective in inhibiting the mycelial growth of the pathogen. In GRAS chemicals, Salicylic acid (2500 ppm), Sodium bicarbonate (5000 ppm) and Potassium sorbate (7500 ppm) resulted in cent per cent inhibition of mycelial growth of the pathogen. Yeasts, botanicals (10%) and GRAS chemicals (2500 ppm) which were found effective under in vitro conditions were further evaluated as fruit protectants under in vivo condition to observe their efficacy against Penicillium digitatum at injured site of Kinnow fruits. Fruits treated at injured site with Saccharomyces cerevisiae BK 6, Ashy Roylea and Salicylic acid resulted in 50, 33.33 and 66.7 per cent reduction in disease incidence, respectively. Similarly, Saccharomyces cerevisiae BK 6, Ashy Roylea and Salicylic acid also reduced the disease severity by 63.3, 33.3 and 78.0 per cent, respectively. Yeasts, GRAS chemicals (2500 ppm) and botanicals (10%) which were found effective under in vitro conditions were further evaluated as fruit dip treatments. Fruits dipped with Saccharomyces cerevisiae R, Salicylic acid and Ashy Roylea resulted in 83.33, 100 and 50 per cent reduction in disease incidence, respectively. Simultaneously, Saccharomyces cerevisiae R, Salicylic acid and Ashy Roylea also reduced the disease severity by 98.67, 100 and 80 per cent, respectively. Saccharomyces cerevisiae treated fruits were found significantly superior over botanicals and GRAS chemicals in improving the quality of Kinnow fruits viz., Titratable acidity (1.01%), TSS (14.6°Brix) and reducing sugars (6.99%). It was also found that the increased content of nitrogen (24.37%), phosphorus (40%), magnesium (66.67%), iron (4.52%), copper (-39.72%), zinc (25.14%) and manganese (80.02%) have positive correlation, whereas potassium (7.32%) and calcium (61.22%) have negative correlation with disease incidence in the treated Kinnow fruits.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810190093
dc.keywordsantagonistic yeasts,, botanicals,GRAS chemicals,Penicillium digitatum Sacc,Kinnow mandarinen_US
dc.language.isoEnglishen_US
dc.pages73+vien_US
dc.publisherUHF,NAUNIen_US
dc.subPlant Pathologyen_US
dc.themeantagonistic yeasts,, botanicals,GRAS chemicals,Penicillium digitatum Sacc,Kinnow mandarinen_US
dc.these.typeM.Scen_US
dc.titlePOTENTIAL OF ANTAGONISTIC YEASTS, BOTANICALS AND GRAS CHEMICALS AGAINST POST-HARVEST ROT CAUSED BY Penicillium digitatum Sacc. IN KINNOW MANDARINen_US
dc.typeThesisen_US
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