Screening and Identification of E. coli pathotypes by Multiplex PCR

Simple item page
dc.contributor.authorAnnal Selva Malar, P
dc.contributor.authorSureshkannan, S
dc.contributor.authorPorteen, K
dc.contributor.authorAsokkumar, M
dc.contributor.authorTANUVAS
dc.date.accessioned2020-03-09T07:28:48Z
dc.date.available2020-03-09T07:28:48Z
dc.date.issued2020-02
dc.descriptionTNV_20thMVC_PP_Feb-2020_PA30en_US
dc.description.abstractThe study was undertaken to screen the E. coli isolates available at Department of Veterinary Public Health and Epidemiology by multiplex PCR to simultaneously group Enterohaemorrhagic E. coli (EHEC), Enteropathogenic E. coli (EPEC) and Enterotoxigenic E. coli (ETEC) pathotypes. A total number of 36 presumptive colonies isolated from milk and various dairy products were confirmed by cultural and biochemical tests according to Bergey’s manual of determinative bacteriology (Holt et al., 1994). The samples found to be positive by culture and biochemical tests were further subjected to DNA extraction by using bio basic molecular biology kit and genus specific PCR analysis was carried out targeting UspA gene (Chen and Griffiths, 1998). The isolates were further analysed for the virulence genes by using multiplex PCR using the primers specific for stx1, stx2, eae, bfp, stII and lt. (Vidal et al.2004). Among the 36 E.coli isolates, three were positive for EPEC (482bp), one was positive for ETEC (129bp) and EHEC (584bp) two were positive for both EPEC and EHEC.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810145036
dc.keywords20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVASen_US
dc.language.isoenen_US
dc.pages1en_US
dc.subjectVeterinary Scienceen_US
dc.titleScreening and Identification of E. coli pathotypes by Multiplex PCRen_US
dc.title.alternativeNational Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safetyen_US
dc.typePresentationen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
TNV_20thMVC_PP_Feb-2020_PA30.pdf
Size:
56.54 KB
Format:
Adobe Portable Document Format
Description:
TNV_20thMVC_PP_Feb-2020_PA30
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Poster Presentations