A Proteomic Approach to Identify Seminal Plasma Proteins in Ostrich (Struthio camelus)

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dc.contributor.authorPandian, C
dc.contributor.authorSelvan, ST
dc.contributor.authorParthiban, M
dc.contributor.authorKarthickeyan, SMK
dc.contributor.authorTANUVAS
dc.date.accessioned2019-01-04T10:26:37Z
dc.date.available2019-01-04T10:26:37Z
dc.date.issued2017-09
dc.descriptionTNV_Ijcmas_2017_6(9)3073-3078en_US
dc.description.abstractThe protein bands in ostrich seminal plasma (OSP) were identified by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI MS) proteomic analysis and compared with NCBI reference sequence. The results revealed that OSP had five major specific proteins bands namely, OSP-I (GATA Zinc finger domain containing protein 1), OSP-II (GATA Zinc finger domain containing protein 1), OSP- III (E3 ubiquitin protein ligase RNF 216, OSP-IV (Mitotic spindle assembly checkpoint protein MAD1) and OSPV (Dual specificity phosphatase DUPD1). The estimated molecular weight of OSP-I (Mr94.51 to 102.34 kDa), OSP-II (Mr75.19 to 93.07 kDa), OSP-III (Mr59.58 to 72.76 kDa), OSP-IV (Mr30.71 to 40.90 kDa) and OSP-V (Mr21.66 to 26.26 kDa). The results of our study provide basic knowledge of the protein composition of ostrich seminal plasma highlighting important physiological pathways which may play crucial roles in the sperm environment after ejaculation. This knowledge can be the basis to further develop procedures improving the reproduction of farmed ostrich.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810088950
dc.keywordsProtein bands, ostrich seminal plasma (OSP)en_US
dc.language.isoenen_US
dc.pages3073-3078en_US
dc.publisherExcellent Publishersen_US
dc.relation.ispartofseries;9
dc.subjectVeterinary Scienceen_US
dc.titleA Proteomic Approach to Identify Seminal Plasma Proteins in Ostrich (Struthio camelus)en_US
dc.title.alternativeInternational Journal of Current Microbiology and Applied Sciencesen_US
dc.typeArticleen_US
dc.volume6en_US
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