Whole genome sequencing and recombination analysis of infectious bronchitis virus (B17 strain)
dc.contributor.author | Shilpa, P | |
dc.contributor.author | Kirubakaran, John | |
dc.contributor.author | Rajasekaran, Ranjani | |
dc.contributor.author | TANUVAS | |
dc.date.accessioned | 2020-03-04T08:42:34Z | |
dc.date.available | 2020-03-04T08:42:34Z | |
dc.date.issued | 2020-02 | |
dc.description | TNV_20thMVC_PP_Feb-2020_OP06 | en_US |
dc.description.abstract | An infectious bronchitis virus - IBV (named as B17 strain) isolated from commercial poultry in an outbreak during 2011, and maintained at the Department of Veterinary Microbiology, Madras Veterinary College was used for the present study. The focus of the present study was to sequence the complete genome, perform phylogenetic analysis for developing a reverse genetics system, which could serve as a platform to provide key insights to the molecular pathogenesis and to develop vaccines. The complete genome of B17 strain of IBV was sequenced as 22 sets of overlapping fragments. The sequences of these fragments were assembled, submitted to GenBank and accession number was obtained (KT203557). Based on comparison of sequences of complete genome of B17 with H120 and H120 variant, the B17 sequence was observed to vary at 50 nucleotides between positions 1 – 25,936 with H120. B17 strain was found to vary with both H120 and H120 variant at the end of the genome between 27,605 and 27,630 a total of 22 nucleotides. Based on phylogenetic tree drawn with complete genome and S1 gene sequences using Maximum likelihood (ML) algorithm with optimum nucleotide distribution model (GTR +G +I ) using the standard strains under genotypes of IBV , the B17 strain grouped in to Mass41 genotype. Further, based on S1 gene sequence alignment matrix, the B17 was observed to vary with H120 vaccine strain only at position 355, wherein the amino acid Glutamine (Q) in H120 was replaced by Glutamic acid (E) in B17. However, no secondary structure difference and recombination sequence was observed in IBV B17. No recombination event was identified in recombination analysis and B17 strain could have possibly evolved from H120 through mutation. | en_US |
dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810144196 | |
dc.keywords | 20th Indian Veterinary Congress, Annual Conference of IAAVR, TANUVAS | en_US |
dc.language.iso | en | en_US |
dc.pages | 1 | en_US |
dc.subject | Veterinary Science | en_US |
dc.title | Whole genome sequencing and recombination analysis of infectious bronchitis virus (B17 strain) | en_US |
dc.title.alternative | National Symposium on Veterinary Research Priorities in Translational Animal Health, Production and Food Safety | en_US |
dc.type | Presentation | en_US |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- TNV_20thMVC_PP_Feb-2020_OP06.pdf
- Size:
- 159.08 KB
- Format:
- Adobe Portable Document Format
- Description:
- TNV_20thMVC_PP_Feb-2020_OP06
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 1.71 KB
- Format:
- Item-specific license agreed upon to submission
- Description: