IDENTIFICATION OF GENOMIC REGIONS CONTROLLING EARLY PLANT VIGOUR IN LENTIL (Lens culinaris Medik.)
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Date
2019-01-07
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CSKHPKV, Palampur
Abstract
The present study was aimed at development of PCR-based linkage map of an F10RIL population of 94 individuals (derived from WA8649090 x Precoz) of lentil and identification of genomic regions linked to early plant vigour traits. Of the 268 (93 SSRs, 3 ISSRs and 172 RAPDs) markers, 265 (90 SSRs, 3 ISSRs and 172 RAPDs) were mapped on seven linkage groups, varying in length between 25.6 to 210.3 cM and covering 809.4 cM with an average marker spacing of 3.05 cM. Number of markers per linkage group varied from 8-76. Seventy one distorted markers were mapped in the linkage map. The study also reported assigning of 24 new cross- genera SSRs of Trifolium pratense on the present linkage map.
Analysis of variance showed considerable genetic variation for all the traits in parental lines and RILs. Shoot length, Root length, Seedling length, No. of leaves and No. of branches showed normal frequency distribution curve whereas Dry weight showed skewed Distribution curve. A compact genomic region (QTL HOTSPOT) consisting of 6 QTLs for Shoot length (cm), Root length (cm) and Seedling length (cm) was observed within a map distance of 56.61 to 86.81 cM on LG1. Some linkage groups showed lack of association with quantitative traits, leading to uneven distribution of QTLs across genome.
The QTLs identified for the traits studied have implications for accelerating the process of pyramiding of favourable genes into adapted genotypes through marker-assisted breeding in lentil. The knowledge of marker-trait association may lead to the identification of genes influencing early plant vigour traits in lentil. Therefore, the PCR- based linkage map and QTL mapping is an effort to provide a basis for MAS in future lentil breeding programs.
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