Pollen storage study in Gladiolus (Gladiolus grandiflorus L.)

dc.contributor.advisorDhatt, K.K.
dc.contributor.authorSimarjit Kaur
dc.date.accessioned2018-02-05T09:26:16Z
dc.date.available2018-02-05T09:26:16Z
dc.date.issued2017
dc.description.abstractThe present investigations entitled ‘Pollen storage study in Gladilous (Gladiolus grandiflorus L.)’ were undertaken in the experimental field area and laboratory of Floriculture and Landscaping, Punjab Agricultural University, Ludhiana, during 2016-17. The experimental material comprised of two varieties ‘Punjab Glance’ as female parent and ‘Yellow Stone’ as male parent. The corms of both the varieties of gladiolus were planted at 15 days interval from October–December to obtain fresh pollen and flowers for pollination. Pollen grains of variety Yellow Stone packed in plastic vials were stored in desiccator at ambient temperature, desiccator with CaCl2 at ambient temperature, desiccator under refrigerator, desiccator with CaCl2 under refrigerator (5˚C), lower cabin under refrigerator (5˚C), freezer (-4°C) for 16 weeks from January-April. Pollen stored under different storage methods was used for determination of pollen viability and pollinating the floret of Punjab Glance along with fresh pollen. Maximum pollen viability was recorded under freezer i.e. 82.87%. Pollen stored under freezer retained pollen viability for longer duration and it was 85.09% after 11weeks and pollen viability decreased to 49.58% after 16 weeks of storage. The capsule setting (61.97%) and number of seeds per capsule (35.42) were maximum when floret pollinated with pollen stored in freezer. Maximum pollen length (29.45µm) and width (15.44µm) obtained under freezer. Pollen of the gladiolus can be stored for longer duration under freezer condition up to 11 weeks of storage without any serious loss of viability. Maximum seed germination (67.12%) was recorded from the seed of flowers pollinated by pollen stored in freezer and it was comparable. The pollen of gladiolus can be stored in freezer (-4˚C) up to 2½ month for obtaining good seed set and pollen viability.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810040396
dc.keywordsPollen, Pollen storage, Pollen viability, Gladiolus grandiflorusen_US
dc.language.isoenen_US
dc.pages53en_US
dc.publisherPunjab Agricultural University, Ludhianaen_US
dc.research.problemPollen storage study in Gladiolus (Gladiolus grandiflorus L.)en_US
dc.subFloriculture and Landscapingen_US
dc.subjectnullen_US
dc.themePollen storage study in Gladiolus (Gladiolus grandiflorus L.)en_US
dc.these.typeM.Scen_US
dc.titlePollen storage study in Gladiolus (Gladiolus grandiflorus L.)en_US
dc.typeThesisen_US
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