Pollen storage study in Gladiolus (Gladiolus grandiflorus L.)
Loading...
Files
Date
2017
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Punjab Agricultural University, Ludhiana
Abstract
The present investigations entitled ‘Pollen storage study in Gladilous (Gladiolus grandiflorus
L.)’ were undertaken in the experimental field area and laboratory of Floriculture and
Landscaping, Punjab Agricultural University, Ludhiana, during 2016-17. The experimental
material comprised of two varieties ‘Punjab Glance’ as female parent and ‘Yellow Stone’ as
male parent. The corms of both the varieties of gladiolus were planted at 15 days interval
from October–December to obtain fresh pollen and flowers for pollination. Pollen grains of
variety Yellow Stone packed in plastic vials were stored in desiccator at ambient temperature,
desiccator with CaCl2 at ambient temperature, desiccator under refrigerator, desiccator with
CaCl2 under refrigerator (5˚C), lower cabin under refrigerator (5˚C), freezer (-4°C) for 16
weeks from January-April. Pollen stored under different storage methods was used for
determination of pollen viability and pollinating the floret of Punjab Glance along with fresh
pollen. Maximum pollen viability was recorded under freezer i.e. 82.87%. Pollen stored under
freezer retained pollen viability for longer duration and it was 85.09% after 11weeks and
pollen viability decreased to 49.58% after 16 weeks of storage. The capsule setting (61.97%)
and number of seeds per capsule (35.42) were maximum when floret pollinated with pollen
stored in freezer. Maximum pollen length (29.45µm) and width (15.44µm) obtained under
freezer. Pollen of the gladiolus can be stored for longer duration under freezer condition up to
11 weeks of storage without any serious loss of viability. Maximum seed germination
(67.12%) was recorded from the seed of flowers pollinated by pollen stored in freezer and it
was comparable. The pollen of gladiolus can be stored in freezer (-4˚C) up to 2½ month for
obtaining good seed set and pollen viability.
Description
Keywords
null