GENETIC DIVERSITY STUDIES USING EST-SSRs AMONG CULTIVARS OF MAIZE (Zea mays L.)

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dc.contributor.advisorDr. N. Sasidharan
dc.contributor.authorARUN RAMCHANDRAN
dc.date.accessioned2017-03-10T08:31:58Z
dc.date.available2017-03-10T08:31:58Z
dc.date.issued2013
dc.description.abstractMaize (Zea mays L) is one of the most important cereals of the world and provides more human food than any other cereal. Maize is grown across all the states in India and in many states; it is one of the important staple foods. Genetic diversity of maize plays a key role in maize breeding. Knowledge of the amount and the distribution of genetic variation within and among maize landraces will provide a guide for predicting the degree of inheritance, variation, and level of heterosis that are essential for maize breeding. In the present study, 28 Maize genotypes were analyzed by 12 EST-derived SSR markers dispersed on 5 of the 10 chromosomes to check genetic diversity among them. The analysis was carried out as per the standard protocol. Twelve primer pairs derived from maize genome produced a total of 76 alleles with an average of 5.91 alleles per primer pair. The dendrogram generated on the principle of Unweighted Pair Wise Method Using Arithmetic Average (UPGMA) was constructed and the genotypes were grouped in to clusters. All the entries of CML series and CM lines clustered into their respective groups. In subcluster A1, GWH-9902 grouped with GWL-1. This is due to the fact that GWH-9902 is one of the parents of GWL-1. The clustering pattern of the A1 sub-cluster revealed that accessions namely GYC-9006 and GYH-9842 clustered along with GWL series accessions. This may be due to accidental mixture of seeds. Also the genotype, Amber grouped separately in this cluster A indicating its aversion from the other lines. Madhuri clustered separately in the second cluster which suggested it is highly diverse from this group of maize lines. The study revealed that EST-derived SSR markers can be efficiently utilized for bringing out the latent diversity existing in maize germplasm belonging to different geographical and agroclimatic regions. Diversity also was observed for maize endosperm colorations which were obscured in some cases, due to admixtures.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810004635
dc.keywordsMAIZE, CULTIVARS, EST-SSRs, GENETIC, DIVERSITYen_US
dc.language.isoenen_US
dc.publisherAAU, Ananden_US
dc.research.problemGENETIC DIVERSITY STUDIES USING EST-SSRs AMONG CULTIVARS OF MAIZE (Zea mays L.)en_US
dc.subAgricultural Biotechnologyen_US
dc.subjectagriculture, biotechnologyen_US
dc.subjectstudyen_US
dc.themeGENETIC DIVERSITY STUDIESen_US
dc.these.typeM.Scen_US
dc.titleGENETIC DIVERSITY STUDIES USING EST-SSRs AMONG CULTIVARS OF MAIZE (Zea mays L.)en_US
dc.typeThesisen_US
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