DEVELOPMENT OF TRANSGENIC CHICKPEA (Cicer arietinum L.) LINES RESISTANT TO Helicoverpa armigera (F.) (LEPIDOPTERA : NOCTUIDAE)
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Date
2009-07-10
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Insect resistant transgenics provide an exciting option as they are likely to reduce
the usage of insecticides for pest management and provide sources of resistance for
deployment. Transgenic chickpea plants were generated through an Agrobacterium
mediated, in planta transformation protocol by incorporating cry1X gene in to KAK-2
variety (strain EHA-105, harbouring the binary vector pBinAR). A tissue cultureindependent
method where in the apical meristem of germinating seedlings is inoculated
and reared into single transformants. These putative T1 transgenics plants were then
selected by adopting multiple evaluation strategies, such as PCR, ELISA and bioassays,
for selection of plants for advancement.
The success rate of PCR is 15.6 per cent in T1 generation was observed. The
associational studies between Cry protein levels expressed in transgenic plants , mean per
cent larval mortalities of Helicoverpa armigera and leaf damage caused by Helicoverpa
asmigera was observed to be non-significant in KAK-2 variety. The western analysis of
PCR-positive plants in T1 generation revealed the presence of the cry protein in the
transgenics.
From the detached leaf bioassays of PCR positive transformants, the per cent leaf
damage caused by Helicoverpa armigera was significantly more in non-transgenic plants
compared to transgenic plants in the KAK-2 variety in the tested generation. It is hoped
that further advancement will help produce the Helicoverpa armigera tolerant and stable
transgenic chickpea variety.
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