HETEROSIS, COMBINING ABILITY AND GENE ACTION STUDIES IN Morus alba L.
Loading...
Date
2022-09
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
UHF,NAUNI
Abstract
ABSTRACT The present investigations entitled “Heterosis, combining ability and gene action studies in Morus alba L.” was carried out in the Department of Tree improvement and Genetic Resources, Dr. Y.S. Parmar University of Horticulture and Forestry Nauni, Solan (HP) during 2019-2022. There is collection of Morus alba genotypes from various states of North India viz; Uttrakhand and J&K. Those were maintained in the clonal orchard from which nine genotypes were included in the study and the plant material of 5 females (S30, Phillipines, K2M5, S41 and M5) and 4 male (China white, S1531, S799 and S1307) genotypes were control crossed using Line x Tester (5 x 4 factorial) mating design. Seedlings of control crosses were raised in the mist chamber and were evaluated for morphological and growth characters. Out of twenty controlled crosses attempted all the crosses successfully produced viable seeds. The per cent successful crosses were highest cross K2M5 × China White (93.33%). Maximum germination percentage (89.47%) was recorded for cross involving K2M5 × S1307. Maximum survival percentage was in Phillipines × S1531 (98.56%). Line × Tester analysis for combining ability revealed that among lines S41 followed by Phillipines and K2M5, and among testers S1307 and China White were found to be good general combiners for most of the characters. On the basis of heterosis over better parent, mean performance and significant desirable SCA effects for morphological characters, the combinations S41 × China White, S41 × S1307 and M5 × China White were found to be the most promising. The gene action study revealed the importance of both additive as well as non-additive gene effects in the inheritance with preponderance of non-
additive gene action. High heritability along with expected genetic gain and high genotypic coefficient of variability were observed for crude protein, nitrogen content and leaf dry weight. These estimates indicated ample opportunities for improvement through selection of best genotypes. Out of 15 ISSR primers three primers were successful in verifying hybridity