ASSESSMENT OF ANTIBIOTIC RESISTANCE PATTERN AND BIOFILM FORMATION IN Pseudomonas aeruginosa ISOLATES FROM BUFFALOES AND DOGS
| dc.contributor.advisor | DEEPIKA KUMARI, G (MAJOR) | |
| dc.contributor.advisor | ANAND KUMAR, P | |
| dc.contributor.advisor | BINDU KIRANMAYI, Ch | |
| dc.contributor.author | RAMA NARAYANA, P | |
| dc.date.accessioned | 2022-10-17T04:36:56Z | |
| dc.date.available | 2022-10-17T04:36:56Z | |
| dc.date.issued | 2022-03 | |
| dc.description | THESES | en_US |
| dc.description.abstract | The current research is conducted to assess the antibiotic resistance pattern and biofilm formation ability in Pseudomonas aeruginosa isolates from the clinical samples of buffaloes and dogs. Samples were inoculated in Brain Heart Infusion (BHI) broth and cultured on Pseudomonas isolation agar. Based on pigment production and biochemical tests (catalase, oxidase, IMViC), 52 samples (34.8%) out of 149 collected in the present study were provisionally confirmed for Pseudomonas species. The isolates were further confirmed by Polymerase Chain Reaction (PCR) assay with Pseudomonas aeruginosa species-specific primers (16S rRNA) and all the 52 isolates yielded specific PCR product of 956 bp. One of the main objectives of the present study, antibiotic resistance pattern was determined by subjecting the PCR confirmed 52 P. aeruginosa isolates to 12 different antibiotic discs in vitro by Kirby Bauer method. The results of the test revealed that co-trimoxazole was highly resistant (98.07%), followed by ampicillin (96.15%), clindamycin (92.30%), oxytetracycline (88.46%) and amoxycillin (84.61%). Streptomycin (75%), ceftriaxone (50%) and norfloxacin (36.53%) were moderately resistant. Ciprofloxacin (5.76%), enrofloxacin (9.61%), amikacin (11.53%) and gentamicin (21.15%) were least resistant than others. The Multiple Antibiotic resistance (MAR) indices of P. aeruginosa isolates ranged from 0.33 to 0.91 in the current study, 51 isolates were confirmed as Multi Drug Resistance (MDR) and 1 isolate was found to be Extensively Drug Resistance (XDR). Phenotypic screening for extended spectrum β-lactamases production was carried by employing phenotypic screening test (PST). Isolates were tested with four cephalosporin antibiotics (cefotaxime, ceftazidime, ceftriaxone, and aztreonam). Forty seven out of the 52 PCR confirmed Pseudomonas aeruginosa isolates were found to be resistant against one or more cephalosporin discs, 44 isolates were resistant against cefotaxime, 26 were resistant against ceftriaxone, 9 were resistant to ceftazidime and 5 against aztreonam. Hence they were considered as ‘suspect ESBL producers’ as defined by the CLSI (2018) guidelines. Thereafter, the suspect ESBL producers were confirmed for Extended Spectrum β Lactamases (ESBL) presence by phenotypic confirmatory tests (PCTs). ESBL production was confirmed in 42 isolates by combination disc method (CDM) and 37 isolates exhibited double disc synergy in double disk synergy test (DDST). Further, all the 52 isolates were tested for genetic determinants of antibiotic resistance with oligonucleotide primers specific for extended spectrum genes (blaOXA, blaSHV, blaTEM). A PCR product of 564 bp, 713 bp and 800 bp size was yielded for blaSHV, blaOXA and blaTEM genes, respectively. Out of 52 isolates of Pseudomonas aeruginosa, 10 isolates were positive only for blaOXA, 9 isolates were positive only for blaSHV, 3 isolates were positive only for blaTEM. Both blaSHV and bla OXA were observed in 5 isolates (9.61 %), both blaSHV and blaTEM were observed in 3 isolates (5.76 %), whereas blaOXA and blaTEM together were observed in 6 isolates (11.50 %). All the three genes blaSHV, blaOXA, blaTEM were observed in 3 isolates (5.67 %). No ESBL genes were observed in 13 isolates. The biofilm formation ability was assessed by processing the P. aeruginosa isolates through congo red agar method (CRA) and tube method. CRA method detected that 7 isolates (13.46%) were weak, 26 (50%) as moderate and 19 (36.53%) isolates to be strong biofilm producers, whereas, in tube method 11 (21.15%) isolates were detected weak, 18 (34.61%) moderate, 23 (44.23%) as strong biofilm producers. In conclusion, the current study investigated the presence of genetic determinants of multidrug resistant P. aeruginosa isolates from clinical samples of buffaloes and dogs in Andhra Pradesh. There is a great necessity to pursue further research on multi-drug and extended drug resistance in Pseudomonas aeruginosa isolates from dairy and companion animals | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810189014 | |
| dc.keywords | ASSESSMENT; ANTIBIOTIC RESISTANCE; PATTERN; BIOFILM FORMATION; Pseudomonas aeruginosa; ISOLATES; BUFFALOES; DOGS | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 87 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Microbiology | en_US |
| dc.theme | ASSESSMENT OF ANTIBIOTIC RESISTANCE PATTERN AND BIOFILM FORMATION IN Pseudomonas aeruginosa ISOLATES FROM BUFFALOES AND DOGS | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | ASSESSMENT OF ANTIBIOTIC RESISTANCE PATTERN AND BIOFILM FORMATION IN Pseudomonas aeruginosa ISOLATES FROM BUFFALOES AND DOGS | en_US |
| dc.type | Thesis | en_US |