BIOPROSPECTING OF COPPER NANOPARTICLES SYNTHESIZING INDIGENOUS BACTERIA AND POTENTIAL APPLICATIONS
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Date
2019-01
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UHF, NAUNI
Abstract
ABSTRACT
Nanotechnology is gaining tremendous incentive in the present century due to its capability of modulating
metals into their nanosize, which changes the chemical, physical and optical properties of metals.The synthesis of
nanostructured materials, especially metallic nanoparticles has gained utmost interest over the few decades owing to
their unique properties that make them applicable in different fields of science and technology.Therefore, isolation
and identification of bacteria with ability of copper nanoparticles synthesis from natural sources is very important in
terms of discovering new industrial products. Keeping in view, copper rich sites located Chambaghat village of
district Solan, Sataun village of Sirmour, Seond and Larji village from Kullu district of Himachal Pradesh and Khetri
Nagar from Rajasthan were selected as a source for new coppernanoparticles synthesizing bacteria. Therefore, aim of
present study was isolation and characterization of copper nanoparticles synthesizing bacteria from these sites for
biosynthesis of copper nanoparticles followed by applications of these copper nanoparticles. A total of 154 putative
copper nanoparticles synthesizing bacterial isolates were obtained from 22 samples collected from different selected
siteswhich were characterized morphologically. Thirty bacterial isolates were selected on the basis of their ability to
show maximum copper nanoparticles synthesizing activity and one bacterial isolate exhibiting maximum copper
nanoparticles synthesizing activity viz., SCS1.1was selected for further molecular characterization using 16S rrna
gene technology. In silico analysis of 16S rrna gene sequence led to identification of this isolate as Stenotrophomonas
maltophilia strain SCS1.1. On the basis of maximum copper nanoparticles synthesizing activity Stenotrophomonas
maltophilia strain SCS1.1 was selected for in vitro biosynthesis of copper nanoparticles. Maximum copper
nanoparticles synthesis was achieved at 30°C, pH 8.0 after 48hrs of incubation with 4.0 mM copper sulphate, 5.0%
peptone, 3.0 % beef extract and 4.0% inoculum size. In vitro biosynthesis of copper nanoparticles was carried out
using optimum conditions which were characterized using UV-visible spectroscopy, FTIR, XRD, TEM, SEM and
DLS. In the present study, ability of copper nanoparticles and bacterial culture suspension of Stenotrophomonas
maltophilia strain SCS1.1. was further assessed for degradation of fifteen textile dyes producing significant results,
these were also found to inhibit various fungal and bacterial pathogens under invitro and in vivo conditions. It has
been observed that 25 ppm of Chlorpyrifos, Profenofos and Imidacloprid were degraded at pH 8 using both copper
nanoparticles and Stenotrophomonas maltophilia strain SCS1.1 culture suspension. Effect of different concentrations
of copper nanoparticles on plant growth and development of twelve crops were studied and 100 ppm were found best
for Solanum lycopersicum and Spinacia oleracea wh
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