“Studies on@-amylase inhibitor (@-AI) in some grain legumes”
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Date
2010
Authors
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Publisher
UHF,NAUNI,SOLAN
Abstract
ABSTRACT
Eighteen different grain legume cultivars of Himalayan region were
analyzed for -amylase inhibitor activity. -Amylase unit inhibited per
gram seed weight was maximum in Hans cultivar of Phaseolus vulgaris L.
-Amylase inhibitor from seeds of Hans cultivar of Phaseolus vulgaris L.
was purified using ammonium sulphate precipitation, gel filtration
chromatography (Sephadex G-200) and ion exchange chromatography
(DEAE-Sephadex). The inhibitor was purified to homogeneity as judged
by native PAGE with 34.42 fold purification and 69.35 per cent recovery.
The purified inhibitor had a molecular weight of 16,218 daltons and was
found to be monomer as revealed by SDS-PAGE. It was found to be heat
stable upto 300C-400C and had two pH optima of 5.0 and 6.9. Nature of
inhibition was found to be of non competitive type as determined by
Lineweaver burk plot. The purified inhibitor was found to have inhibitory
activity against -amylases extracted from larvae of Callosobruchus
chinensis, Tribolium castaneum and mid gut of Corcyra cephalonica.
Purified -amylase inhibitor was also found to inhibit human salivary -
amylase.
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