FORMULATION AND EFFECTIVENESS OF FLUID BED DRIED CONSORTIA OF AGRICULTURALLY BENEFICIAL MICROORGANISMS
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Date
2014-09-10
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University of Agricultural Sciences GKVK, Bangalore
Abstract
This study focused on formulation development using suitable carrier material in
a fluid bed dryer (FBD) of different agriculturally beneficial microorganisms, survival of
these inoculant formulation prepared in fluid bed dryer at different intervals and
effectiveness of these inoculant formulation on cow pea (Vigna unguiculata L.) and
finger millet (Eleusine coracana Gaertn.) crop. Among different carriers (flyash, talc and
skim milk powder) tried in different combinations, formulation prepared usig a
combination of talc and skim milk powder at 40° C in FBD resulted in better
survivability of microbial population. The two consortia using Rhizobium sp., Bacillus
megaterium and Pseudomonas fluorescens and another consortia of Acinetobacter sp.,
Bacillus subtilis and Azotobacter chroococcum was prepared using talc and skim milk
powder in a fluid bed dryer. The survival of these two consortia was monitored during
360 and 365 days of storage period respectively where in the triple inoculant formulation
prepared using FBD resulted in maximum cell survival till the end of storage period. No
contamination was observed in all FBD inoculant formulation at the end of 180 days at
10-5 dilution. Effectiveness of Rhizobium sp., Bacillus megaterium and Pseudomonas
fluorescens on cowpea; Acinetobacter sp., Bacillus subtilis and Azotobacter chroococcum
on finger millet crop was carried out in green house conditions at two nutrient levels with
lignite based formulation as control. It was observed that both cowpea and finger millet
plants inoculated with fluid bed dried triple inoculant formulation of both consortia under
the influence of nutrients showed better plant growth parameters, higher nitrogen and
phosphorus uptake followed by dual and single inoculants when compared to lignite
based formulations.
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