SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS AND EXPRESSION PROFILE OF INHIBIN ALPHA GENE IN MALABARI GOATS

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dc.contributor.advisorR. Thirupathy Venkatachalapathy
dc.contributor.authorHARITHA B. PILLAI
dc.date.accessioned2020-09-23T07:16:23Z
dc.date.available2020-09-23T07:16:23Z
dc.date.issued2019
dc.description.abstractThe present study was carried out to identify single nucleotide variations in inhibin alpha (INHA) gene to evaluate its association with production traits and find out the tissue expression profile in Malabari goats. A total of 207 Malabari adult goats were screened for polymorphism using PCR-SSCP. Representative samples of each SSCP pattern were sequenced to ascertain the genotypes. Five fragments, 274 bp, 220 bp, 160 bp, 287 bp and 253 bp covering the two exons of INHA were analysed. Similar monomorphic band pattern was observed for 220 bp fragment of exon 2. The 274 bp fragment showed two diplotypes (AA and AB) and sequencing revealed one synonymous SNP (c.129G>A) in exon 1. The AA genotype showed significant (p≤0.05) association with body length. The 160 bp fragment exhibited two genotypes DD and DE with a synonymous SNP c.567G>A in exon 2. Goats carrying DD genotype had significantly (p≤0.05) higher adult body weight and body length compared to DE genotype. Three diplotypes, HH, HJ and JJ with a synonymous SNP, c.651G>A were observed at 287 bp fragment. Significantly (p<0.01) higher body length was noticed in goats with HJ and JJ genotypes. The 253 bp fragment of exon 2 exhibited three genotypes PP, PQ and QQ with a non synonymous SNP, c.911T>C. Genotypes of INHA 253 bp (exon 2) locus had significant (p≤0.05) influence on body weight and trunk circumference. The PP and PQ genotypes showed a significantly higher trunk circumference (p≤0.05) compared to QQ. Tissue samples of ovary, uterus and fallopian tube were collected from seven Malabari adult goats to study INHA gene expression pattern. The relative tissue expressions of INHA mRNA were normalised with the reference gene (GAPDH). Relative expression was significantly (p<0.01) higher in ovary, followed by uterus and fallopian tube. The results obtained from this study indicate the potential role of INHA gene on reproduction and growth; hence it can be used as a candidate gene for the genetic improvement of Malabari goats.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151829
dc.keywordsSINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS AND EXPRESSION PROFILE OF INHIBIN ALPHA GENE IN MALABARI GOATSen_US
dc.language.isoEnglishen_US
dc.pages98en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemSINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS AND EXPRESSION PROFILE OF INHIBIN ALPHA GENE IN MALABARI GOATSen_US
dc.subAnimal Genetics and Breedingen_US
dc.themeSINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS AND EXPRESSION PROFILE OF INHIBIN ALPHA GENE IN MALABARI GOATSen_US
dc.these.typeM.V.Sc.en_US
dc.titleSINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS AND EXPRESSION PROFILE OF INHIBIN ALPHA GENE IN MALABARI GOATSen_US
dc.typeThesisen_US
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