SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS AND EXPRESSION PROFILE OF INHIBIN ALPHA GENE IN MALABARI GOATS
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Date
2019
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College of Veterinary and animal Science,Mannuthy
Abstract
The present study was carried out to identify single nucleotide variations in
inhibin alpha (INHA) gene to evaluate its association with production traits and find
out the tissue expression profile in Malabari goats. A total of 207 Malabari adult
goats were screened for polymorphism using PCR-SSCP. Representative samples of
each SSCP pattern were sequenced to ascertain the genotypes. Five fragments, 274
bp, 220 bp, 160 bp, 287 bp and 253 bp covering the two exons of INHA were
analysed. Similar monomorphic band pattern was observed for 220 bp fragment of
exon 2. The 274 bp fragment showed two diplotypes (AA and AB) and sequencing
revealed one synonymous SNP (c.129G>A) in exon 1. The AA genotype showed
significant (p≤0.05) association with body length. The 160 bp fragment exhibited
two genotypes DD and DE with a synonymous SNP c.567G>A in exon 2. Goats
carrying DD genotype had significantly (p≤0.05) higher adult body weight and body
length compared to DE genotype. Three diplotypes, HH, HJ and JJ with a
synonymous SNP, c.651G>A were observed at 287 bp fragment. Significantly
(p<0.01) higher body length was noticed in goats with HJ and JJ genotypes. The 253
bp fragment of exon 2 exhibited three genotypes PP, PQ and QQ with a non
synonymous SNP, c.911T>C. Genotypes of INHA 253 bp (exon 2) locus had
significant (p≤0.05) influence on body weight and trunk circumference. The PP and
PQ genotypes showed a significantly higher trunk circumference (p≤0.05) compared
to QQ. Tissue samples of ovary, uterus and fallopian tube were collected from seven
Malabari adult goats to study INHA gene expression pattern. The relative tissue
expressions of INHA mRNA were normalised with the reference gene (GAPDH).
Relative expression was significantly (p<0.01) higher in ovary, followed by uterus
and fallopian tube. The results obtained from this study indicate the potential role of
INHA gene on reproduction and growth; hence it can be used as a candidate gene for
the genetic improvement of Malabari goats.